Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Xanthobacter sp. 91 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.06 | - |
NAD+ | at pH 7.0 and 25°C | Xanthobacter sp. 91 | |
9 | - |
formate | with NAD+ as cosubstrate, at pH 7.0 and 25°C | Xanthobacter sp. 91 | |
37 | - |
NADP+ | at pH 7.0 and 25°C | Xanthobacter sp. 91 | |
100 | - |
formate | with NADP+ as cosubstrate, at pH 7.0 and 25°C | Xanthobacter sp. 91 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Xanthobacter sp. 91 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
formate + NAD+ | - |
Xanthobacter sp. 91 | CO2 + NADH + H+ | - |
? | |
formate + NADP+ | - |
Xanthobacter sp. 91 | CO2 + NADPH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
FDH | - |
Xanthobacter sp. 91 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Xanthobacter sp. 91 |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.4 | - |
NADP+ | at pH 7.0 and 25°C | Xanthobacter sp. 91 | |
0.4 | - |
formate | with NADP+ as cosubstrate, at pH 7.0 and 25°C | Xanthobacter sp. 91 | |
31 | - |
NAD+ | at pH 7.0 and 25°C | Xanthobacter sp. 91 | |
31 | - |
formate | with NAD+ as cosubstrate, at pH 7.0 and 25°C | Xanthobacter sp. 91 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
the enzyme activity decreases upon pH increases | Xanthobacter sp. 91 |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | the enzyme prefers to use NAD+ as an electron acceptor more than NADP+ | Xanthobacter sp. 91 | |
NADP+ | the enzyme prefers to use NAD+ as an electron acceptor more than NADP+ | Xanthobacter sp. 91 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0019 | - |
formate | with NADP+ as cosubstrate, at pH 7.0 and 25°C | Xanthobacter sp. 91 | |
0.011 | - |
NADP+ | at pH 7.0 and 25°C | Xanthobacter sp. 91 | |
3 | - |
formate | with NAD+ as cosubstrate, at pH 7.0 and 25°C | Xanthobacter sp. 91 | |
479 | - |
NAD+ | at pH 7.0 and 25°C | Xanthobacter sp. 91 |