Literature summary for 1.17.1.9 extracted from

Altas, N.; Aslan, A.; Karatas, E.; Chronopoulou, E.; Labrou, N.; Binay, B.
Heterologous production of extreme alkaline thermostable NAD+-dependent formate dehydrogenase with wide-range pH activity from Myceliophthora thermophila (2017), Proc. Biochem., 61, 110-118 .

No PubMed abstract available

Search for more literature for 1.17.1.9

Application

Application	Comment	Organism
biofuel production	potential applications in NAD(H)-dependent industrial biocatalysis as well as in the production of renewable fuels and chemicals from carbon dioxide. Formate dehydrogenase from Myceliophthora thermophile possess a huge potential for CO2 reduction or NADH generation and under extreme alkaline conditions	Thermothelomyces thermophilus
synthesis	potential applications in NAD(H)-dependent industrial biocatalysis as well as in the production of renewable fuels and chemicals from carbon dioxide. Formate dehydrogenase from Myceliophthora thermophile possess a huge potential for CO2 reduction or NADH generation and under extreme alkaline conditions	Thermothelomyces thermophilus

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli, as 6His-tagged protein	Thermothelomyces thermophilus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.18	-	NADH	25°C, pH not specified in the publication	Thermothelomyces thermophilus
0.43	-	CO2	25°C, pH not specified in the publication	Thermothelomyces thermophilus
1.2	-	NAD+	25°C, pH not specified in the publication	Thermothelomyces thermophilus
7.2	-	formate	25°C, pH not specified in the publication	Thermothelomyces thermophilus

Organism

Organism	UniProt	Comment	Textmining
Thermothelomyces thermophilus	G2QB71	-	-
Thermothelomyces thermophilus ATCC 42464	G2QB71	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Thermothelomyces thermophilus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
formate + NAD+	-	Thermothelomyces thermophilus	CO2 + NADH + H+	-	r
formate + NAD+	-	Thermothelomyces thermophilus ATCC 42464	CO2 + NADH + H+	-	r

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
35	-	-	Thermothelomyces thermophilus

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
25	49	25°C: about 75% of maximal activity, 49°C: about 40% of maximal activity	Thermothelomyces thermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
48	-	Tm-value	Thermothelomyces thermophilus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.03	-	NADH	25°C, pH not specified in the publication	Thermothelomyces thermophilus
0.1	-	CO2	25°C, pH not specified in the publication	Thermothelomyces thermophilus
0.32	-	formate	25°C, pH not specified in the publication	Thermothelomyces thermophilus
0.76	-	NAD+	25°C, pH not specified in the publication	Thermothelomyces thermophilus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
5.5	-	conversion of carbon dioxide to formate using as cofactor NADH	Thermothelomyces thermophilus
10.5	-	conversion of formate to carbon dioxide	Thermothelomyces thermophilus

Cofactor

Cofactor	Comment	Organism	Structure
NAD+	-	Thermothelomyces thermophilus
NADH	-	Thermothelomyces thermophilus

pI Value

Organism	Comment	pI Value Maximum	pI Value
Thermothelomyces thermophilus	calculated from sequence	-	6.15

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
0.04	-	formate	25°C, pH not specified in the publication	Thermothelomyces thermophilus
0.17	-	NADH	25°C, pH not specified in the publication	Thermothelomyces thermophilus
0.23	-	CO2	25°C, pH not specified in the publication	Thermothelomyces thermophilus
0.63	-	NAD+	25°C, pH not specified in the publication	Thermothelomyces thermophilus

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Release 2023.1 (January 2023)