Crystallization (Comment) | Organism |
---|---|
re-evaluation of crystallographic data | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P07658 | isoform H | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
formate + NAD+ = CO2 + NADH + H+ | reaction mechanism: step I, formate binds directly to Mo, displacing Se-Cys140. Step II, the alpha-proton from formate may be transferred to the nearby His141 that acts as general base. Alternatively, step II may involve a selenium-carboxylated intermediate. Step III, electrons from MoIV are transferred via the 4Fe-4S center to an external electron acceptor and the catalytic cycle is completed | Escherichia coli |