Literature summary for 1.17.1.9 extracted from

Ansorge-Schumacher, M.B.; Slusarczyk, H.; Schuemers, J.; Hirtz, D.
Directed evolution of formate dehydrogenase from Candida boidinii for improved stability during entrapment in polyacrylamide (2006), FEBS J., 273, 3938-3945.

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Protein Variants

Protein Variants	Comment	Organism
E53V	90% residual activity in presence of 8% acrylamide and 0.1% TEMED, 4fold increase compared to wild-type	[Candida] boidinii
E53V/K56R	90% residual activity in presence of 8% acrylamide and 0.1% TEMED, 4.1fold increase compared to wild-type	[Candida] boidinii
E53V/K56R/C23S	90% residual activity in presence of 15% acrylamide and 0.1% TEMED, 42fold increase compared to wild-type	[Candida] boidinii
K35T	70% residual activity in presence of 8% acrylamide and 0.1% TEMED, 2.7fold increase compared to wild-type	[Candida] boidinii

Organism

Organism	UniProt	Comment	Textmining
[Candida] boidinii	-	isolation of mutants with increased residual activity after entrapment in polyacrylamide gels	-

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Release 2023.1 (January 2023)