Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP-beta-D-ribose | competitive with NAD+, mixed-type with formate | Gelatoporia subvermispora | |
azide | uncompetitive with NAD+, competitive with formate | Gelatoporia subvermispora | |
bicarbonate | mixed-type inhibition of NAD+ and formate | Gelatoporia subvermispora | |
Hg2+ | strong inhibition | Gelatoporia subvermispora | |
iodoacetate | strong inhibition | Gelatoporia subvermispora | |
additional information | not inhibitory: Mg2+, Mn2+, Co2+, Ni2+, Cd2+, Zn2+, Ca2+, Fe2+, Fe3+, Cu2+ | Gelatoporia subvermispora | |
NADH | competitive with NADH, mixed-type with formate | Gelatoporia subvermispora | |
NADP+ | strong inhibition | Gelatoporia subvermispora | |
NADPH | strong inhibition | Gelatoporia subvermispora | |
p-chloromercuribenzoate | strong inhibition | Gelatoporia subvermispora |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.028 | - |
NAD+ | pH 6.5, 30°C | Gelatoporia subvermispora | |
2.5 | - |
formate | pH 6.5, 30°C | Gelatoporia subvermispora |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
2 * 42000, SDS-PAGE | Gelatoporia subvermispora |
84000 | - |
gel filtration | Gelatoporia subvermispora |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Gelatoporia subvermispora | possible role of enzyme in oxalate metabolism | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gelatoporia subvermispora | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Gelatoporia subvermispora |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
formate + NAD+ = CO2 + NADH + H+ | ordered BiBi mechanism with NAD+ and NADH being the first substrate and the second product, resp. | Gelatoporia subvermispora |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
383.4 | - |
pH 6.5, 30°C | Gelatoporia subvermispora |
Storage Stability | Organism |
---|---|
activity is retained in presence of dithiothreitol for 6 months, but lost within weeks in absence of SH compounds | Gelatoporia subvermispora |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
formate + NAD+ | - |
Gelatoporia subvermispora | CO2 + NADH + H+ | - |
? | |
additional information | possible role of enzyme in oxalate metabolism | Gelatoporia subvermispora | ? | - |
? | |
additional information | no substrate: acetate, pyruvate, malate, oxalate, succinate, methanol, isocitrate, fumarate | Gelatoporia subvermispora | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 42000, SDS-PAGE | Gelatoporia subvermispora |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 30 | - |
Gelatoporia subvermispora |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Gelatoporia subvermispora |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | specific for NAD+ as electron acceptor | Gelatoporia subvermispora |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0000239 | - |
azide | substrate formate, pH 6.5, 30°C | Gelatoporia subvermispora | |
0.00095 | - |
azide | substrate NAD+, pH 6.5, 30°C | Gelatoporia subvermispora | |
0.0228 | - |
ADP-beta-D-ribose | substrate formate, pH 6.5, 30°C | Gelatoporia subvermispora | |
0.0369 | - |
ADP-beta-D-ribose | substrate NAD+, pH 6.5, 30°C | Gelatoporia subvermispora | |
0.053 | - |
bicarbonate | substrate formate, pH 6.5, 30°C | Gelatoporia subvermispora | |
0.06 | - |
NADH | substrate NAD+, pH 6.5, 30°C | Gelatoporia subvermispora | |
1.2 | - |
bicarbonate | substrate formate, pH 6.5, 30°C | Gelatoporia subvermispora | |
5.6 | - |
bicarbonate | substrate NAD+, pH 6.5, 30°C | Gelatoporia subvermispora |