Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Mycolicibacterium vaccae |
Protein Variants | Comment | Organism |
---|---|---|
A198G/D221Q | the mutant has the highest catalytic efficiency with NADP+ | Mycolicibacterium vaccae |
C145S/A198G/D221Q/C255V | the mutant has high catalytic efficiency with NADP+ | Mycolicibacterium vaccae |
C145S/D221A/C255V | the mutant has high catalytic efficiency with NADP+ | Mycolicibacterium vaccae |
C145S/D221Q/C255V | the mutant shows the highest specific activity for a NADP+-accepting formate dehydrogenase | Mycolicibacterium vaccae |
D221A | the mutant has high catalytic efficiency with NADP+ | Mycolicibacterium vaccae |
D221G | the mutant has high catalytic efficiency with NADP+ | Mycolicibacterium vaccae |
D221Q | the mutant has high catalytic efficiency with NADP+ | Mycolicibacterium vaccae |
D221S | the mutant has high catalytic efficiency with NADP+ | Mycolicibacterium vaccae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ethyl 4-chloroacetoacetate | - |
Mycolicibacterium vaccae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.084 | - |
NADP+ | mutant C145S/D221Q/C255V, at pH 7.0 and 22°C | Mycolicibacterium vaccae | |
0.147 | - |
NADP+ | mutant C145S/A198G/D221Q/C255V, at pH 7.0 and 22°C | Mycolicibacterium vaccae | |
0.24 | - |
NADP+ | mutant D221G/C255V, at pH 7.0 and 22°C | Mycolicibacterium vaccae | |
0.27 | - |
NADP+ | mutant A198G/D221Q, at pH 7.0 and 22°C | Mycolicibacterium vaccae | |
0.31 | - |
NADP+ | mutant D221G, at pH 7.0 and 22°C | Mycolicibacterium vaccae | |
0.37 | - |
NADP+ | mutant D221A, at pH 7.0 and 22°C | Mycolicibacterium vaccae | |
0.39 | - |
NADP+ | mutant D221Q, at pH 7.0 and 22°C | Mycolicibacterium vaccae | |
0.43 | - |
NADP+ | mutant D221S, at pH 7.0 and 22°C | Mycolicibacterium vaccae | |
0.57 | - |
NADP+ | mutant C145S/D221G/C255V, at pH 7.0 and 22°C | Mycolicibacterium vaccae | |
0.92 | - |
NADP+ | mutant C145S/D221A/C255V, at pH 7.0 and 22°C | Mycolicibacterium vaccae | |
2 | 3 | formate | mutant D221A, at pH 7.0 and 22°C | Mycolicibacterium vaccae | |
40 | - |
formate | mutant D221G, at pH 7.0 and 22°C | Mycolicibacterium vaccae | |
41 | - |
formate | mutant D221S, at pH 7.0 and 22°C | Mycolicibacterium vaccae | |
46 | - |
formate | mutant D221Q, at pH 7.0 and 22°C | Mycolicibacterium vaccae | |
62 | - |
formate | mutant D221G/C255V, at pH 7.0 and 22°C | Mycolicibacterium vaccae | |
69 | - |
formate | mutant C145S/D221G/C255V, at pH 7.0 and 22°C | Mycolicibacterium vaccae | |
127 | - |
formate | mutant A198G/D221Q, at pH 7.0 and 22°C | Mycolicibacterium vaccae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
formate + NADP+ | Mycolicibacterium vaccae | - |
CO2 + NADPH | - |
? | |
formate + NADP+ | Mycolicibacterium vaccae N10 | - |
CO2 + NADPH | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycolicibacterium vaccae | - |
- |
- |
Mycolicibacterium vaccae N10 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
HisTrap column chromatography | Mycolicibacterium vaccae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
formate + NADP+ | - |
Mycolicibacterium vaccae | CO2 + NADPH | - |
? | |
formate + NADP+ | - |
Mycolicibacterium vaccae N10 | CO2 + NADPH | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.52 | - |
NADP+ | mutant D221G, at pH 7.0 and 22°C | Mycolicibacterium vaccae | |
0.55 | - |
NADP+ | mutant D221S, at pH 7.0 and 22°C | Mycolicibacterium vaccae | |
0.62 | - |
NADP+ | mutant D221G/C255V, at pH 7.0 and 22°C | Mycolicibacterium vaccae | |
0.79 | - |
NADP+ | mutant A198G/D221Q, at pH 7.0 and 22°C | Mycolicibacterium vaccae | |
1.04 | - |
NADP+ | mutant D221Q, at pH 7.0 and 22°C | Mycolicibacterium vaccae | |
1.05 | - |
NADP+ | mutant D221A, at pH 7.0 and 22°C | Mycolicibacterium vaccae | |
1.5 | - |
NADP+ | mutant C145S/D221Q/C255V, at pH 7.0 and 22°C | Mycolicibacterium vaccae | |
1.64 | - |
NADP+ | mutant C145S/D221G/C255V, at pH 7.0 and 22°C | Mycolicibacterium vaccae | |
3.08 | - |
NADP+ | mutant C145S/A198G/D221Q/C255V, at pH 7.0 and 22°C | Mycolicibacterium vaccae | |
7.89 | - |
NADP+ | mutant C145S/D221A/C255V, at pH 7.0 and 22°C | Mycolicibacterium vaccae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Mycolicibacterium vaccae |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.29 | - |
NADP+ | mutant D221S, at pH 7.0 and 22°C | Mycolicibacterium vaccae | |
1.69 | - |
NADP+ | mutant D221G, at pH 7.0 and 22°C | Mycolicibacterium vaccae | |
2.58 | - |
NADP+ | mutant D221G/C255V, at pH 7.0 and 22°C | Mycolicibacterium vaccae | |
2.67 | - |
NADP+ | mutant D221Q, at pH 7.0 and 22°C | Mycolicibacterium vaccae | |
2.83 | - |
NADP+ | mutant D221A, at pH 7.0 and 22°C | Mycolicibacterium vaccae | |
2.88 | - |
NADP+ | mutant C145S/D221G/C255V, at pH 7.0 and 22°C | Mycolicibacterium vaccae | |
2.94 | - |
NADP+ | mutant A198G/D221Q, at pH 7.0 and 22°C | Mycolicibacterium vaccae | |
8.58 | - |
NADP+ | mutant C145S/D221A/C255V, at pH 7.0 and 22°C | Mycolicibacterium vaccae | |
17.9 | - |
NADP+ | mutant C145S/D221Q/C255V, at pH 7.0 and 22°C | Mycolicibacterium vaccae | |
21 | - |
NADP+ | mutant C145S/A198G/D221Q/C255V, at pH 7.0 and 22°C | Mycolicibacterium vaccae |