General Stability | Organism |
---|---|
azide stabilizes during purification | Moorella thermoacetica |
dithionite stabilizes during purification | Moorella thermoacetica |
glycerol stabilizes during purification | Moorella thermoacetica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.109 | - |
formate | - |
Moorella thermoacetica | |
0.117 | - |
NADP+ | - |
Moorella thermoacetica |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | 36 mol of iron per mol of enzyme | Moorella thermoacetica | |
selenium | 2 mol of selenium per mol of enzyme, resides in the alpha-subunit | Moorella thermoacetica | |
Tungsten | 2 mol of tungsten per mol of enzyme | Moorella thermoacetica | |
Tungsten | may exist as tungsten cofactor | Moorella thermoacetica |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
76000 | - |
alpha2 beta2, 2 * 96000 + 2 * 76000 SDS-PAGE | Moorella thermoacetica |
96000 | - |
alpha2 beta2, 2 * 96000 + 2 * 76000 SDS-PAGE | Moorella thermoacetica |
340000 | - |
gel filtration | Moorella thermoacetica |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Moorella thermoacetica | - |
- |
- |
Oxidation Stability | Organism |
---|---|
extremely oxygen-sensitive | Moorella thermoacetica |
Purification (Comment) | Organism |
---|---|
- |
Moorella thermoacetica |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1050 | - |
- |
Moorella thermoacetica |
Storage Stability | Organism |
---|---|
4°C, anaerobic conditions, glycerol, ammonium sulfate, 45 days, 35% loss of activity | Moorella thermoacetica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CO2 + NADPH | enzyme also catalyzes: 1. reversible electron transfer between methyl viologen and NADPH, 2. reduction of FMN or FAD with NADPH (caused by a contaminating protein) | Moorella thermoacetica | formate + NADP+ | - |
? | |
CO2 + NADPH | reduction of FMN or FAD with NADPH caused by a contaminating protein | Moorella thermoacetica | formate + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | alpha2 beta2, 2 * 96000 + 2 * 76000 SDS-PAGE | Moorella thermoacetica |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Moorella thermoacetica | |
NADPH | - |
Moorella thermoacetica |