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Literature summary for 1.17.1.10 extracted from
- Formate dehydrogenase, a selenium-tungsten enzyme from Clostridium thermoaceticum (1970), Methods Enzymol., 53, 360-372.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| sulfhydryl compounds | required for activity, e.g. dithiothreitol, cysteine, glutathione, thioglycolate | Moorella thermoacetica |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2,3-Butanedione | sodium azide and NADP+ protect | Moorella thermoacetica | |
| azide | competitive inhibitor | Moorella thermoacetica | |
| cyanide | 50% inhibition at 0.01 mM, 100% inhibition at 10 mM | Moorella thermoacetica | |
| EDTA | 50% inhibition at 25 mM, 100% inhibition at 100 mM | Moorella thermoacetica | |
| hypophosphite | competitive inhibitor | Moorella thermoacetica | |
| mercaptoethanol | - |
Moorella thermoacetica | |
| additional information | enzyme is rapidly inactivated by oxygen: 93% loss of activity within 2 min | Moorella thermoacetica |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.02 | - |
NADPH | - |
Moorella thermoacetica | |
| 0.083 | - |
formate | cosubstrate methyl viologen | Moorella thermoacetica | |
| 0.11 | - |
NADP+ | - |
Moorella thermoacetica | |
| 0.23 | - |
formate | cosubstrate NADP+ | Moorella thermoacetica | |
| 2.35 | - |
reduced methyl viologen | - |
Moorella thermoacetica | |
| 11 | - |
CO2 | - |
Moorella thermoacetica | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Molybdenum | - |
Moorella thermoacetica | |
| selenium | - |
Moorella thermoacetica | |
| Tungsten | - |
Moorella thermoacetica | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 270000 | 300000 | gel filtration | Moorella thermoacetica |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CO2 + NADPH | Moorella thermoacetica | first enzyme in pathway of reduction of CO2 to acetate: CO2 serves as electron acceptor generated during fermentation | formate + NADP+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Moorella thermoacetica | - |
- |
- |
Oxidation Stability
| Oxidation Stability | Organism |
|---|---|
| rapid inactivation by air, thiol-iron complexes and formate stabilize | Moorella thermoacetica |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Moorella thermoacetica |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 36.6 | - |
- |
Moorella thermoacetica |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CO2 + NADPH | enzyme also catalyzes: 3. oxidation of NADPH with O2 | Moorella thermoacetica | formate + NADP+ | - |
r | |
| CO2 + NADPH | also reacts with: methyl viologen, benzyl viologen | Moorella thermoacetica | formate + NADP+ | - |
r | |
| CO2 + NADPH | first enzyme in pathway of reduction of CO2 to acetate: CO2 serves as electron acceptor generated during fermentation | Moorella thermoacetica | formate + NADP+ | - |
? | |
| formate + reduced methyl viologen | - |
Moorella thermoacetica | CO2 + oxidized methyl viologen | - |
r | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
formate + NADP+ | Moorella thermoacetica |
| 85 | - |
formate + methyl viologen | Moorella thermoacetica |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
low activity below | Moorella thermoacetica |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
3 h, slight loss of activity | Moorella thermoacetica |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
broad, CO2 reduction | Moorella thermoacetica |
| 7 | 9.5 | formate oxidation | Moorella thermoacetica |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | - |
Moorella thermoacetica | |
| NADPH | - |
Moorella thermoacetica | |
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Release 2023.1 (January 2023)
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