Application | Comment | Organism |
---|---|---|
biotechnology | expression system is developped producing about 2 mg of purified Bacillus sp. strain PL-12 Mn(II) oxidase per liter of Escherichia coli culture in 5 days | Bacillus sp. (in: Bacteria) |
Cloned (Comment) | Organism |
---|---|
the mnxDEFG operon construct is expressed without a tag in Escherichia coli by inducing at 17°C and loading with 2 mM CuSO4 under microaerobic conditions | Bacillus sp. (in: Bacteria) |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus sp. (in: Bacteria) | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using a series of native chromatography steps: hydrophobic interaction, gel filtration, and ion exchange | Bacillus sp. (in: Bacteria) |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4 Mn(II) + 4 H+ + O2 | - |
Bacillus sp. (in: Bacteria) | 4 Mn(III) + 2 H2O | - |
? | |
4 Mn(III) + 4 H+ + O2 | - |
Bacillus sp. (in: Bacteria) | 4 Mn(IV) + 2 H2O | - |
? | |
additional information | Mn oxidation from soluble Mn(II) to Mn(IV) oxides is a two-step reaction catalyzed by an MCO-containing complex | Bacillus sp. (in: Bacteria) | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
mnxDEFG | - |
Bacillus sp. (in: Bacteria) |
multicopper oxidase | - |
Bacillus sp. (in: Bacteria) |