Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
purified recombinant H ferritin, 10 mg/ml protein in 10 mM Tris,HCl, pH 7.5, and 0.15 M NaCl, is mixed at equal volumes with crystallization solution containing 0.1 M BICINE-Na, pH 9.0, and 1.9-2.0 M MgCl2, 20°C, 1 week, X-ray diffraction structure determination and analysis at 1.52-1.97 A resolution | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
E140A | site-directed mutagenesis, the initial velocity of iron oxidization is reduced in the mutant | Homo sapiens |
E140Q | site-directed mutagenesis, the initial velocity of iron oxidization is highly reduced in the mutant. The side chain of the mutated Gln140 is fixed by a hydrogen bond, whereas that of native Glu140 is flexible | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | stopped-flow kinetics and initial velocities of iron oxidation of wild-type and mutant enzymes, overview | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P02794 | ferritin heavy chain | - |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by heat treatment at 60°C for 10 min, followed by ammonium sulfate fractionation, anion exchange chromatography, and gel filtration | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | enzyme metal binding structure, overview | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | three-dimensional structural analysis of wild-type and mutant H ferritins, overview | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
H ferritin | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
additional information | ferritin is a ubiquitous iron storage protein that possesses ferroxidase activity | Homo sapiens |