Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Bacillus sp. (in: Bacteria) |
Protein Variants | Comment | Organism |
---|---|---|
Y264F | Km-value for Hg2+ is 5fold lower compared to the Km-value of the wild-type enzyme, turn-over number is reduced by 164fold | Bacillus sp. (in: Bacteria) |
Y264F/Y605F | Km-value for Hg2+ is 5fold lower than the Km-value of the wild-type enzyme, turnover-number is reduced by 1091fold | Bacillus sp. (in: Bacteria) |
Y605F | Km-value for Hg2+ is 1.3fold higher compared to the Km-value of the wild-type enzyme, turnover-number is reduced by 6.3fold | Bacillus sp. (in: Bacteria) |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
Hg2+ | - |
Bacillus sp. (in: Bacteria) |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
69000 | - |
x * 69000, SDS-PAGE | Bacillus sp. (in: Bacteria) |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus sp. (in: Bacteria) | - |
- |
- |
Bacillus sp. (in: Bacteria) RC607 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Bacillus sp. (in: Bacteria) |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Hg2+ + NADPH | - |
Bacillus sp. (in: Bacteria) | Hg + NADP+ + H+ | - |
? | |
Hg2+ + NADPH | - |
Bacillus sp. (in: Bacteria) RC607 | Hg + NADP+ + H+ | - |
? | |
additional information | Tyr264 and Tyr605 are involved in substrate binding, Tyr264 is important for catalysis, possibly by destabilizing the binding of Hg(II) to the two ligating thiolates at the active site | Bacillus sp. (in: Bacteria) | ? | - |
? | |
additional information | Tyr264 and Tyr605 are involved in substrate binding, Tyr264 is important for catalysis, possibly by destabilizing the binding of Hg(II) to the two ligating thiolates at the active site | Bacillus sp. (in: Bacteria) RC607 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 69000, SDS-PAGE | Bacillus sp. (in: Bacteria) |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
12 | - |
Hg2+ | - |
Bacillus sp. (in: Bacteria) |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Bacillus sp. (in: Bacteria) |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Bacillus sp. (in: Bacteria) |