Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Acetylene | mechanism-based inhibitor, specifically interacts with catalytically active ammonia monooxygenase | Nitrosomonas europaea |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | the enzyme resides in the cytoplasm of the bacteria in addition to its location in the membrane and is distributed approximately equally in both subcellular fractions | Nitrosomonas europaea | 5737 | - |
membrane | the enzyme resides in the cytoplasm of the bacteria in addition to its location in the membrane and is distributed approximately equally in both subcellular fractions | Nitrosomonas europaea | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | the enzyme contains Cu (9.4 mol per mol enzyme), Fe (3.9 mol per mol enzyme), and Zn (0.5 to 2.6 mol per mol enzyme) | Nitrosomonas europaea | |
Iron | the enzyme contains Cu (9.4 mol per mol enzyme), Fe (3.9 mol per mol enzyme), and Zn (0.5 to 2.6 mol per mol enzyme) | Nitrosomonas europaea | |
Zinc | the enzyme contains Cu (9.4 mol per mol enzyme), Fe (3.9 mol per mol enzyme), and Zn (0.5 to 2.6 mol per mol enzyme) | Nitrosomonas europaea |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
24000 | - |
3 * 27000 (alpha-subunit, AmoA) + 3 * 42000 (beta-subunit, AmoB) + 3 * 24000 (gamma-subunit, cytochrome c1), soluble enzyme, SDS-PAGE | Nitrosomonas europaea |
27000 | - |
3 * 27000 (alpha-subunit, AmoA) + 3 * 42000 (beta-subunit, AmoB) + 3 * 24000 (gamma-subunit, cytochrome c1), soluble enzyme, SDS-PAGE | Nitrosomonas europaea |
42000 | - |
3 * 27000 (alpha-subunit, AmoA) + 3 * 42000 (beta-subunit, AmoB) + 3 * 24000 (gamma-subunit, cytochrome c1), soluble enzyme, SDS-PAGE | Nitrosomonas europaea |
283000 | - |
non-denaturing gel electrophoresis | Nitrosomonas europaea |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ammonia + AH2 + O2 | Nitrosomonas europaea | - |
NH2OH + A + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nitrosomonas europaea | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Nitrosomonas europaea |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.089 | - |
intact cells | Nitrosomonas europaea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ammonia + AH2 + O2 | - |
Nitrosomonas europaea | NH2OH + A + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | different from the beta-subunit of membrane-bound ammonia monooxygenase, the beta-subunit of soluble ammonia monooxygenase possesses an N-terminal signal sequence | Nitrosomonas europaea |
nonamer | 3 * 27000 (alpha-subunit, AmoA) + 3 * 42000 (beta-subunit, AmoB) + 3 * 24000 (gamma-subunit, cytochrome c1), soluble enzyme, SDS-PAGE | Nitrosomonas europaea |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome c1 | the gamma-subunit of the alpha3beta3gamma3 enzyme is cytochrome c1 | Nitrosomonas europaea |