Cloned (Comment) | Organism |
---|---|
gene EhDES15, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenic analysis, a codon-optimized EhDES15 gene lacking the 74 amino acid peptide serving as putative bipartite signal and transit peptides at the N-terminus is synthesized and expressed heterologously Synechocystis sp. PCC 6803 cells under control of the trc promoter leading to increased level of n-3 fatty acid species,which are produced at only low levels in wild-type cyanobacterial cells grown at 30°C, semi-quantitative PCR expression analysis. Expression of gene EhDES15 in a Synechocystis mutant lacking the desB gene causes accumulation of 18:3n-3 and 18:4n-3 in the transformed cells | Emiliania huxleyi |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Emiliania huxleyi | 9507 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Emiliania huxleyi | R1FY33 | - |
- |
Emiliania huxleyi CCMP1516 | R1FY33 | - |
- |
Synonyms | Comment | Organism |
---|---|---|
DELTA15 desaturase | - |
Emiliania huxleyi |
DELTA15 fatty acid desaturase | - |
Emiliania huxleyi |
EhDES15 | - |
Emiliania huxleyi |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme is is thought to be involved in 18:5n-3 biosynthesis | Emiliania huxleyi |
additional information | the enzyme contains a putative transit sequence for localization in chloroplasts and a DELTA6 desaturase-like domain, but it does not contain a cytochrome b5 domain nor typical His-boxes found in DELTA15 desaturases | Emiliania huxleyi |