Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ascorbate | absolutely dependent on | Pyrus communis | |
CO2 | absolutely dependent on | Pyrus communis | |
CO2 | best at 14% or higher | Pyrus communis | |
O2 | dependent on | Pyrus communis | |
Triton X-100 | best at 0.8% or above | Pyrus communis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
alpha-aminoisobutyric acid | - |
Pyrus communis | |
Co2+ | - |
Pyrus communis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.057 | - |
1-aminocyclopropane-1-carboxylate | pH 6.7, 30°C | Pyrus communis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | absolutely required | Pyrus communis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-aminocyclopropane-1-carboxylate + ascorbate + O2 | Pyrus communis | - |
ethylene + cyanide + dehydroascorbate + CO2 + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrus communis | - |
cv.Blanquilla | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fruit | - |
Pyrus communis | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0047 | - |
crude enzyme extract | Pyrus communis |
0.006 | - |
pear fruit prior homogenization | Pyrus communis |
Storage Stability | Organism |
---|---|
-20°C, 30% glycerol, stable for 11 days | Pyrus communis |
2°C, 30% glycerol, loss of 30% activity after 24 h | Pyrus communis |
2°C, 30% glycerol, loss of 85% activity after 7 h | Pyrus communis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-aminocyclopropane-1-carboxylate + ascorbate + O2 | - |
Pyrus communis | ethylene + cyanide + dehydroascorbate + CO2 + H2O | - |
? | |
1-aminocyclopropane-1-carboxylate + L-ascorbate + O2 | absolutely dependent on CO2 | Pyrus communis | ethylene + cyanide + dehydroascorbate + CO2 + H2O | - |
? | |
1-aminocyclopropane-1-carboxylate + L-ascorbate + O2 | i.e. ACC | Pyrus communis | ethylene + cyanide + dehydroascorbate + CO2 + H2O | - |
? | |
1-aminocyclopropane-1-carboxylate + L-ascorbate + O2 | absolutely dependent on ascorbate | Pyrus communis | ethylene + cyanide + dehydroascorbate + CO2 + H2O | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
28 | - |
2 optima at 28°C and 38°C | Pyrus communis |
38 | - |
2 optima at 28°C and 38°C | Pyrus communis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
4.2 | - |
alpha-aminoisobutyric acid | pH 6.7, 30°C | Pyrus communis |