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Literature summary for 1.14.17.4 extracted from

  • Zhang, Z.H.; Barlow, J.N.; Baldwin, J.E.; Schofield, C.J.
    Metal-catalyzed oxidation and mutagenesis studies on the iron(II) binding site of 1-aminocyclopropane-1-carboxylate oxidase (1997), Biochemistry, 36, 15999-16007.
    View publication on PubMed
Search for more literature for 1.14.17.4

Cloned(Commentary)

Cloned (Comment) Organism
wild-type enzyme and mutants, expression in Escherichia coli BL21(DE3) Solanum lycopersicum

Protein Variants

Protein Variants Comment Organism
D179E site-directed mutagenesis, very low activity, stimulation by bicarbonate Solanum lycopersicum
D179N site-directed mutagenesis, no activity Solanum lycopersicum
H177D site-directed mutagenesis, no activity Solanum lycopersicum
H177D/D179E site-directed mutagenesis, no activity Solanum lycopersicum
H177E site-directed mutagenesis, very low activity, stimulation by bicarbonate Solanum lycopersicum
H177Q site-directed mutagenesis, no activity Solanum lycopersicum
H211Q site-directed mutagenesis, slightly reduced activity Solanum lycopersicum
H234D site-directed mutagenesis, no activity Solanum lycopersicum
H234E site-directed mutagenesis, no activity Solanum lycopersicum
H234Q site-directed mutagenesis, no activity Solanum lycopersicum
H39Q site-directed mutagenesis, slightly reduced activity Solanum lycopersicum
H56Q site-directed mutagenesis, activity similar to the wild-type Solanum lycopersicum
H94Q site-directed mutagenesis, slightly reduced activity Solanum lycopersicum

General Stability

General Stability Organism
enzyme has a short half-life under catalytic conditions undergoing metal-catalyzed oxidative fragmentation Solanum lycopersicum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00029
-
 Fe2+ recombinant mutant H177E, in presence of 5 mM L-ascorbate and 10 mM HCO3-, pH 7.2, 28°C Solanum lycopersicum
0.00043
-
 Fe2+ recombinant wild-type enzyme, in presence of 5 mM L-ascorbate and 10 mM HCO3-, pH 7.2, 28°C Solanum lycopersicum
0.00047
-
 Fe2+ recombinant mutant H56Q, in presence of 5 mM L-ascorbate and 10 mM HCO3-, pH 7.2, 28°C Solanum lycopersicum
0.0046
-
 Fe2+ recombinant mutant D179E, in presence of 5 mM L-ascorbate and 10 mM HCO3-, pH 7.2, 28°C Solanum lycopersicum
0.042
-
 1-aminocyclopropane-1-carboxylate recombinant mutant H177E, in presence of 5 mM L-ascorbate and 10 mM HCO3-, pH 7.2, 28°C Solanum lycopersicum
0.083
-
 1-aminocyclopropane-1-carboxylate recombinant wild-type enzyme, in presence of 5 mM L-ascorbate and 10 mM HCO3-, pH 7.2, 28°C Solanum lycopersicum
0.121
-
 1-aminocyclopropane-1-carboxylate recombinant mutant H56Q, in presence of 5 mM L-ascorbate and 10 mM HCO3-, pH 7.2, 28°C Solanum lycopersicum
0.132
-
 1-aminocyclopropane-1-carboxylate recombinant mutant D179E, in presence of 5 mM L-ascorbate and 10 mM HCO3-, pH 7.2, 28°C Solanum lycopersicum

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ required Solanum lycopersicum
Fe2+ iron binding ligands are His177, Asp179, His234 Solanum lycopersicum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1-aminocyclopropane-1-carboxylate + ascorbate + O2 Solanum lycopersicum biosynthesis of plant signalling molecule ethylene ethylene + cyanide + dehydroascorbate + CO2 + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Solanum lycopersicum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant wild-type enzyme and mutants from Escherichia coli BL21(DE3) to near homogeneity Solanum lycopersicum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-aminocyclopropane-1-carboxylate + ascorbate + O2 biosynthesis of plant signalling molecule ethylene Solanum lycopersicum ethylene + cyanide + dehydroascorbate + CO2 + H2O
-
 ?
1-aminocyclopropane-1-carboxylate + L-ascorbate + O2 i.e. ACC Solanum lycopersicum ethylene + cyanide + dehydroascorbate + CO2 + H2O
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
28
-
 assay at Solanum lycopersicum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00867
-
 1-aminocyclopropane-1-carboxylate recombinant mutant D179E, in presence of 5 mM L-ascorbate and 10 mM HCO3-, pH 7.2, 28°C Solanum lycopersicum
0.0095
-
 1-aminocyclopropane-1-carboxylate recombinant mutant H177E, in presence of 5 mM L-ascorbate and 10 mM HCO3-, pH 7.2, 28°C Solanum lycopersicum
5.17
-
 1-aminocyclopropane-1-carboxylate recombinant mutant H56Q, in presence of 5 mM L-ascorbate and 10 mM HCO3-, pH 7.2, 28°C Solanum lycopersicum
5.9
-
 1-aminocyclopropane-1-carboxylate recombinant wild-type enzyme, in presence of 5 mM L-ascorbate and 10 mM HCO3-, pH 7.2, 28°C Solanum lycopersicum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
 assay at Solanum lycopersicum