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Literature summary for 1.14.17.4 extracted from

  • Lay, V.J.; Prescott, A.G.; Thomas, P.G.; John, P.
    Heterologous expression and site-directed mutagenesis of the 1-aminocyclopropane-1-carboxylate oxidase from kiwi fruit (1996), Eur. J. Biochem., 242, 228-234.
    View publication on PubMed
Search for more literature for 1.14.17.4

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and mutants in Escherichia coli Actinidia deliciosa

Protein Variants

Protein Variants Comment Organism
D179E site-directed mutagenesis, 1% activity compared to the recombinant wild-type enzyme Actinidia deliciosa
G137P site-directed mutagenesis, 98% activity compared to the recombinant wild-type enzyme Actinidia deliciosa
H177Q site-directed mutagenesis, 1% activity compared to the recombinant wild-type enzyme Actinidia deliciosa
K158A site-directed mutagenesis, 1% activity compared to the recombinant wild-type enzyme Actinidia deliciosa
K158C site-directed mutagenesis, 1% activity compared to the recombinant wild-type enzyme Actinidia deliciosa
K172A site-directed mutagenesis, 85% activity compared to the recombinant wild-type enzyme Actinidia deliciosa
K172C site-directed mutagenesis, 64% activity compared to the recombinant wild-type enzyme Actinidia deliciosa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1-aminocyclopropane-1-carboxylate + ascorbate + O2 Actinidia deliciosa
-
 ethylene + cyanide + dehydroascorbate + CO2 + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Actinidia deliciosa P31237 kiwi
-

Purification (Commentary)

Purification (Comment) Organism
recombinant wild-type enzyme and mutants from Escherichia coli Actinidia deliciosa

Source Tissue

Source Tissue Comment Organism Textmining
fruit
-
 Actinidia deliciosa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.073
-
 purified recombinant mutant K172C Actinidia deliciosa
0.1
-
 purified recombinant mutant K172A Actinidia deliciosa
0.11
-
 purified recombinant mutant G137P Actinidia deliciosa
0.16
-
 purified recombinant wild-type enzyme Actinidia deliciosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-aminocyclopropane-1-carboxylate + ascorbate + O2
-
 Actinidia deliciosa ethylene + cyanide + dehydroascorbate + CO2 + H2O
-
 ?
1-aminocyclopropane-1-carboxylate + L-ascorbate + O2 i.e. ACC Actinidia deliciosa ethylene + cyanide + dehydroascorbate + CO2 + H2O
-
 ?