Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ascorbate | required | Solanum lycopersicum | |
ascorbate | cannot be replaced by DTT or NADH | Solanum lycopersicum | |
bovine serum albumin | stimulates the recombinant enzyme by 41% at 0.1 mM | Solanum lycopersicum | |
catalase | recombinant enzyme, stimulates by 36% at 0.5 mg/ml | Solanum lycopersicum | |
CO2 | requires HCO3-/CO2 mixture | Solanum lycopersicum | |
dithiothreitol | recombinant enzyme, stimulates by 38% at 1 mM | Solanum lycopersicum | |
ethylene | stimulates | Solanum lycopersicum |
Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli BL21(DE3) | Solanum lycopersicum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
diethyl dicarbonate | complete inactivation at 1.5 mM, pH 6.8, after 20 min | Solanum lycopersicum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0053 | - |
Fe2+ | recombinant enzyme, in presence of 5 mM L-ascorbate and 10 mM HCO3-, pH 7.2, 28°C | Solanum lycopersicum | |
0.032 | - |
1-aminocyclopropane-1-carboxylate | recombinant enzyme, in presence of 5mM L-ascorbate and 10 mM HCO3-, pH 7.2, 28°C | Solanum lycopersicum | |
1 | - |
5,6-O-isopropylidine L-ascorbate | recombinant enzyme, in presence of 5 mM L-ascorbate and 10 mM HCO3-, pH 7.2, 28°C | Solanum lycopersicum | |
1.7 | - |
D-ascorbate | recombinant enzyme, in presence of 5 mM L-ascorbate and 10 mM HCO3-, pH 7.2, 28°C | Solanum lycopersicum | |
16.7 | - |
L-ascorbate | recombinant enzyme, in presence of 5 mM L-ascorbate and 10 mM HCO3-, pH 7.2, 28°C | Solanum lycopersicum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35820 | - |
electron spray mass spectrometry | Solanum lycopersicum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-aminocyclopropane-1-carboxylate + ascorbate + O2 | Solanum lycopersicum | - |
ethylene + cyanide + dehydroascorbate + CO2 + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Solanum lycopersicum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant from E. coli, 9.8fold to near homogeneity | Solanum lycopersicum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.035 | - |
purified enzyme | Solanum lycopersicum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-amino-2-ethylcyclopropane-1-carboxylate + L-ascorbate + O2 | prefers mixture of 1R,2S- and 1S,2R-amino-2-ethylcyclopropane-1-carboxylate, less effective with racemic mixture of 1R,2R- and 1S,2S-diastereomers | Solanum lycopersicum | 1-butene + cyanide + dehydroascorbate + CO2 + H2O | - |
? | |
1-aminocyclopropane-1-carboxylate + 5,6-O-isopropylidine L-ascorbate + O2 | better cosubstrate than L-ascorbate | Solanum lycopersicum | ethylene + cyanide + 5,6-O-isopropylidine L-dehydroascorbate + CO2 + H2O | - |
? | |
1-aminocyclopropane-1-carboxylate + ascorbate + O2 | - |
Solanum lycopersicum | ethylene + cyanide + dehydroascorbate + CO2 + H2O | - |
? | |
1-aminocyclopropane-1-carboxylate + D-ascorbate + O2 | better cosubstrate than L-ascorbate | Solanum lycopersicum | ethylene + cyanide + D-dehydroascorbate + CO2 + H2O | - |
? | |
1-aminocyclopropane-1-carboxylate + L-ascorbate + O2 | i.e. ACC | Solanum lycopersicum | ethylene + cyanide + dehydroascorbate + CO2 + H2O | - |
? | |
additional information | L-galactono-gamma-lactone is no cosubstrate | Solanum lycopersicum | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
28 | - |
assay at | Solanum lycopersicum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
assay at | Solanum lycopersicum |