Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-amino-4-hydroxy-6,7-dimethyl-tetrahydropteridine | pteridine cofactor required, 2-amino-4-hydroxy-6,7-dimethyl-tetrahydropteridine is most effective, optimal concentration is 0.000025 mM | Pseudomonas putida | |
tetrahydrofolic acid | pteridine cofactor required, 50% of the activity of 2-amino-4-hydroxy-6,7-dimethyl-tetrahydropteridine | Pseudomonas putida |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | 0.5 mM, 88% inhibition | Pseudomonas putida | |
2,2'-bipyridyl | 0.5 mM, 83% inhibition | Pseudomonas putida | |
2-amino-4-hydroxy-6,7-dimethyl-tetrahydropteridine | above 0.000025 mM, slight inhibition | Pseudomonas putida | |
4-hydroxymercuribenzoate | partially reversible by thiol compounds | Pseudomonas putida | |
8-hydroxyquinoline | 0.5 mM, 60% inhibition | Pseudomonas putida | |
Ag2+ | 0.0001 mM, 42% inhibition | Pseudomonas putida | |
amethopterin | - |
Pseudomonas putida | |
Aminopterine | - |
Pseudomonas putida | |
Cd2+ | 0.0001 mM, 66% inhibition | Pseudomonas putida | |
Cu2+ | 0.0001 mM, 40% inhibition | Pseudomonas putida | |
diethyldithiocarbamate | 0.5 mM, 16% inhibition | Pseudomonas putida | |
EDTA | 0.5 mM, 64% inhibition | Pseudomonas putida | |
guanidine hydrochloride | 2.0 mM, 95% inhibition | Pseudomonas putida | |
Hg2+ | 0.0001 mM, 56% inhibition | Pseudomonas putida | |
iodoacetamide | weak | Pseudomonas putida | |
iodoacetate | weak | Pseudomonas putida | |
NEM | - |
Pseudomonas putida | |
SDS | 0.1 mM, complete inhibition | Pseudomonas putida | |
Thiourea | 1.0 mM, complete inhibition | Pseudomonas putida | |
Urea | 2.0 mM, complete inhibition | Pseudomonas putida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
(S)-2-Hydroxy-2-phenylacetate | - |
Pseudomonas putida | |
0.19 | - |
NADPH | - |
Pseudomonas putida |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | required | Pseudomonas putida | |
Fe2+ | Km: 0.047 mM | Pseudomonas putida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
91000 | - |
gel filtration | Pseudomonas putida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-2-hydroxy-2-phenylacetate + tetrahydropteridine + O2 | Pseudomonas putida | i.e. L-mandelate, first step of oxidative degradation of L-mandelate by Pseudomonas convexa | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas putida |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.021 | - |
- |
Pseudomonas putida |
Storage Stability | Organism |
---|---|
-20°C, partially purified enzyme is stable for 36-40 h | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-2-hydroxy-2-phenylacetate + tetrahydropteridine | i.e. L-mandelate, highly substrate specific, D-mandelate is not hydroxylated | Pseudomonas putida | (S)-4-hydroxymandelate + dihydropteridine + H2O | - |
? | |
(S)-2-hydroxy-2-phenylacetate + tetrahydropteridine + O2 | i.e. L-mandelate, first step of oxidative degradation of L-mandelate by Pseudomonas convexa | Pseudomonas putida | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
38 | - |
- |
Pseudomonas putida |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
5 min, no loss of activity | Pseudomonas putida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.4 | - |
citrate-phosphate buffer | Pseudomonas putida |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.6 | 7.4 | half-maximal activity at pH 4.6 and pH 7.4 | Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | required | Pseudomonas putida |