KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0344 | - |
6,7-dimethyl-5,6,7,8-tetrahydropterin | enzyme form II, substrate L-phenylalanine | Rattus norvegicus | |
0.037 | - |
6-Methyl-5,6,7,8-tetrahydropterin | enzyme form I, substrate L-phenylalanine | Rattus norvegicus | |
0.0444 | - |
6,7-dimethyl-5,6,7,8-tetrahydropterin | enzyme form I, substrate L-phenylalanine | Rattus norvegicus | |
0.0455 | - |
6-Methyl-5,6,7,8-tetrahydropterin | enzyme form II, substrate L-phenylalanine | Rattus norvegicus | |
0.454 | - |
L-phenylalanine | cofactor 6-methyl-5,6,7,8-tetrahydropterin, enzyme form II | Rattus norvegicus | |
0.72 | - |
L-phenylalanine | cofactor 6-methyl-5,6,7,8-tetrahydropterin, enzyme form I | Rattus norvegicus | |
1.43 | - |
L-phenylalanine | cofactor 6,7-dimethyl-5,6,7,8-tetrahydropterin, enzyme form II | Rattus norvegicus | |
1.92 | - |
L-phenylalanine | cofactor 6,7-dimethyl-5,6,7,8-tetrahydropterin, enzyme form I | Rattus norvegicus | |
4.9 | - |
L-tryptophan | cofactor 2-amino-4-hydroxy-6-methyltetrahydropteridine | Rattus norvegicus | |
8.5 | - |
L-tryptophan | cofactor 2-amino-4-hydroxy-6,7-dimethyltetrahydropteridine, enzyme form I | Rattus norvegicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | iron-protein | Rattus norvegicus | |
Iron | 0.6 mol of iron per mol of 51000 Da subunit | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
51000 | - |
4 * 51000, enzyme form I and II, SDS-PAGE | Rattus norvegicus |
240000 | - |
sedimentation equilibrium | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
side-chain modification | enzyme form I contains 0.3 mol phosphate per mol subunit, enzyme form II contains 0.4 mol | Rattus norvegicus |
Purification (Comment) | Organism |
---|---|
distinct forms I and II differing in phosphate content and isoelectric point | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.59 | - |
- |
Rattus norvegicus |
1.46 | - |
- |
Rattus norvegicus |
1.46 | - |
enzyme form II | Rattus norvegicus |
1.8 | - |
enzyme form I | Rattus norvegicus |
Storage Stability | Organism |
---|---|
-80°C, 1 month, 30% loss of activity | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-phenylalanine + tetrahydrobiopterin + O2 | - |
Rattus norvegicus | L-tyrosine + dihydrobiopterin + H2O | - |
? | |
L-tryptophan + tetrahydrobiopterin + O2 | - |
Rattus norvegicus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 51000, enzyme form I and II, SDS-PAGE | Rattus norvegicus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
9.08 | - |
phenylalanine | cofactor 6,7-dimethyl-5,6,7,8-tetrahydropterin | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
tetrahydrobiopterin | - |
Rattus norvegicus |