General Stability | Organism |
---|---|
inclusion into phospholipid vesicles stabilizes | Bos taurus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | cytochrome P-45011beta in inner mitochondrial membrane, adrenodoxin reductase and adrenodoxin are loosely associated, inclusion of purified P-45011beta into phospholipid vesicles mimics the situation in vivo | Bos taurus | 5739 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
48000 | - |
x * 48000, SDS-PAGE | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, octyl-Sepharose adsorption with cholate and phosphotidylcholine extraction | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
adrenal cortex | - |
Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.35 | 1.36 | corticosterone, enzyme incorporated into liposomes | Bos taurus |
1.38 | - |
corticosterone, soluble form of enzyme | Bos taurus |
Storage Stability | Organism |
---|---|
4°C, enzyme incorporated into liposomes, stable during 2 weeks | Bos taurus |
4°C, soluble form, loses part of activity in few days, after 2 weeks 1/4 of original activity observed | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
11-deoxycorticosterone + reduced adrenal ferredoxin + O2 | 11beta-hydroxylation | Bos taurus | corticosterone + oxidized adrenal ferredoxin + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 48000, SDS-PAGE | Bos taurus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Bos taurus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Bos taurus |