Application | Comment | Organism |
---|---|---|
synthesis | expression of PdmAB in Escherichia coli, Pseudomonas putida, and other sphingomonads results in a functional enzyme. Coexpression of a putative [3Fe-4S]-type ferredoxin from Sphingomonas sp. strain RW1 greatly enhances the catalytic activity of PdmAB in Escherichia coli | Sphingobium sp. YBL2 |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Sphingobium sp. YBL2 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sphingobium sp. YBL2 | S5RR11 and S5SC42 | S5RR11 i.e. subunit PudmA, S5SC42 i.e. subunit PudmB | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
chlorotoluron + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 | i.e. herbicide N'-(3-chloro-4-methylphenyl)-N,N-dimethylurea | Sphingobium sp. YBL2 | N-(3-chloro-4-methylphenyl)-N'-methylurea + formaldehyde + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? | |
diuron + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 | - |
Sphingobium sp. YBL2 | N-(3,4-dichlorophenyl)-N'-methylurea + formaldehyde + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? | |
fenuron + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 | i.e. herbicide N,N-dimethyl-N'-phenylurea | Sphingobium sp. YBL2 | N-methyl-N'-phenylurea + formaldehyde + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? | |
fluometuron + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 | i.e. herbicide N,N-dimethyl-N'-[3-(trifluoromethyl)phenyl]urea | Sphingobium sp. YBL2 | N-methyl-N'-[3-(trifluoromethyl)phenyl]urea + formaldehyde + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? | |
isoproturon + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 | i.e. herbicide N,N-dimethyl-N'-[4-(propan-2-yl)phenyl]urea | Sphingobium sp. YBL2 | N-methyl-N'-[4-(propan-2-yl)phenyl]urea + formaldehyde + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? | |
metoxuron + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 | i.e. herbicide N'-(3-chloro-4-methoxyphenyl)-N,N-dimethylurea | Sphingobium sp. YBL2 | N'-(3-chloro-4-methoxyphenyl)-N-methylurea + formaldehyde + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? | |
monuron + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 | i.e. herbicide N'-(4-chlorophenyl)-N,N-dimethylurea | Sphingobium sp. YBL2 | N-(4-chlorophenyl)-N'-methylurea + formaldehyde + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O | - |
? | |
additional information | Escherichia coli cell suspensions expressing PudmAB can convert all the N,N-dimethyl-substituted phenylurea herbicides tested to their mono-N-demethylated derivatives but show no detectable activity for N-methoxy-N-methyl-substituted ones | Sphingobium sp. YBL2 | ? | - |
- |
Synonyms | Comment | Organism |
---|---|---|
PudmA | - |
Sphingobium sp. YBL2 |
PudmB | - |
Sphingobium sp. YBL2 |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | PdmAB has a low specificity for electron transport components and its optimal ferredoxin may be the [3Fe-4S] type | Sphingobium sp. YBL2 |
General Information | Comment | Organism |
---|---|---|
physiological function | the pdmAB genes are responsible for the initial mono-N-demethylation of N,N-dimethyl-substituted phenylurea herbicides. PdmAB is the oxygenase component of a bacterial Rieske non-heme iron oxygenase system. A subunit PudmA disruption strain does not show the ability to degrade isoproturon and other N,N-dimethyl substituted phenylurea herbicides, but it can still degrade the known metabolites of isoproturon | Sphingobium sp. YBL2 |