Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, CYP17 genotyping, realtime PCR expression analysis | Ovis aries |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0016 | - |
progesterone | isozyme 1, pH 7.4, temperature not specified in the publication | Ovis aries | |
0.0017 | - |
pregnenolone | isozyme 1, pH 7.4, temperature not specified in the publication | Ovis aries | |
0.0018 | - |
progesterone | isozyme 2, pH 7.4, temperature not specified in the publication | Ovis aries | |
0.0019 | - |
pregnenolone | isozyme 2, pH 7.4, temperature not specified in the publication | Ovis aries |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Ovis aries | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Ovis aries | the bifunctional enzyme catalyzes two types of reactions, namely, a 17alpha-hydroxylation reaction and a 17,20-lyase reaction | ? | - |
? | |
pregnenolone + [reduced NADPH-hemoprotein reductase] + O2 | Ovis aries | - |
17alpha-hydroxypregnenolone + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
progesterone + [reduced NADPH-hemoprotein reductase] + O2 | Ovis aries | - |
17alpha-hydroxyprogesterone + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ovis aries | Q29497 | GenBank accession numbers L40335 and AF251388 encodíng isozymes 1 and 2; two isozymes of cytochrome P450 17alpha-hydroxylase/17,20-lyase, that differ in two nucleotides, resulting in variants Ser210Gly and Tyr464Asn | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
adrenal cortex | - |
Ovis aries | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the bifunctional enzyme catalyzes two types of reactions, namely, a 17alpha-hydroxylation reaction and a 17,20-lyase reaction | Ovis aries | ? | - |
? | |
pregnenolone + [reduced NADPH-hemoprotein reductase] + O2 | - |
Ovis aries | 17alpha-hydroxypregnenolone + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
progesterone + [reduced NADPH-hemoprotein reductase] + O2 | - |
Ovis aries | 17alpha-hydroxyprogesterone + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CYP17 | - |
Ovis aries |
cytochrome P450 17alpha-hydroxylase/17,20-lyase | - |
Ovis aries |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Ovis aries |