Cloned (Comment) | Organism |
---|---|
expression in monkey kidney COS-1 cells | Cavia porcellus |
expression in monkey kidney COS-1 cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
R200N | increased reactivity towards pregnenolone, converts pregnenolone to 17alpha-hydroxypregnenolone and dehydroepiandrosterone, at the expense of 17,20-lyase activity towards 17alpha-hydroxyprogesterone | Cavia porcellus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00008 | - |
pregnenolone | wild type enzyme | Cavia porcellus | |
0.00022 | - |
pregnenolone | R200N mutant | Cavia porcellus | |
0.00024 | - |
progesterone | wild type enzyme | Cavia porcellus | |
0.00025 | - |
pregnenolone | - |
Homo sapiens | |
0.00031 | - |
progesterone | R200N mutant | Cavia porcellus | |
0.00045 | - |
progesterone | - |
Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Homo sapiens | - |
- |
microsome | membrane-bound | Cavia porcellus | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
progesterone + [reduced NADPH-hemoprotein reductase] + O2 | Cavia porcellus | - |
17alpha-hydroxyprogesterone + [oxidized NADPH-hemoprotein reductase] + H2O | product eliminates at C20,21 acetate to yield androstenedione | ? | |
progesterone + [reduced NADPH-hemoprotein reductase] + O2 | Homo sapiens | - |
17alpha-hydroxyprogesterone + [oxidized NADPH-hemoprotein reductase] + H2O | product eliminates at C20,21 acetate to yield androstenedione | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cavia porcellus | - |
- |
- |
Homo sapiens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
progesterone + [reduced NADPH-hemoprotein reductase] + O2 | - |
Cavia porcellus | 17alpha-hydroxyprogesterone + [oxidized NADPH-hemoprotein reductase] + H2O | product eliminates at C20,21 acetate to yield androstenedione | ? | |
progesterone + [reduced NADPH-hemoprotein reductase] + O2 | - |
Homo sapiens | 17alpha-hydroxyprogesterone + [oxidized NADPH-hemoprotein reductase] + H2O | product eliminates at C20,21 acetate to yield androstenedione | ? |