Literature summary for 1.14.14.182 extracted from

Chau, M.; Jennewein, S.; Walker, K.; Croteau, R.
Taxol biosynthesis: Molecular cloning and characterization of a cytochrome P450 taxoid 7beta-hydroxylase (2004), Chem. Biol., 11, 663-672.

Search for more literature for 1.14.14.182

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in a Spodoptera fugiperda-baculovirus-based expression system	Taxus cuspidata

General Stability

General Stability	Organism
taxhydroxylase activity is slightly higher in HEPES than in Tris-HCl, however, hydrolytic activity (enzymatic deacylation of both substrate and product) is also more prominent in HEPES7	Taxus cuspidata

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0076	-	(+)-taxusin	pH 7.5, temperature not specified in the publication	Taxus cuspidata

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
microsome	-	Taxus cuspidata	-	-

Organism

Organism	UniProt	Comment	Textmining
Taxus cuspidata	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
P-93AF cell	-	Taxus cuspidata	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(+)-taxusin + O2 + NADPH + H+	i.e. taxadien-5alpha,9alpha,10beta,13alpha-tetraol tetraacetate	Taxus cuspidata	7beta-hydroxytaxusin + NADP+ + H2O	-	?

Synonyms

Synonyms	Comment	Organism
T7H	-	Taxus cuspidata
taxoid 7beta-hydroxylase	-	Taxus cuspidata

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	-	Taxus cuspidata

Cofactor

Cofactor	Comment	Organism	Structure
cytochrome P450	-	Taxus cuspidata
NADPH	-	Taxus cuspidata

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Release 2023.1 (January 2023)