Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.14.13.92 extracted from

  • Parshin, P.D.; Pometun, A.A.; Martysuk, U.A.; Kleymenov, S.Y.; Atroshenko, D.L.; Pometun, E.V.; Savin, S.S.; Tishkov, V.I.
    Effect of His6-tag position on the expression and properties of phenylacetone monooxygenase from Thermobifida fusca (2020), Biochemistry (Moscow), 85, 575-582 .
    View publication on PubMed

Application

Application Comment Organism
synthesis phenylacetone monooxygenase (PAMO) catalyzes oxidation of ketones with molecular oxygen and NADPH with the formation of esters. PAMO demonstrates high catalytic constants in the oxidation of benzylacetone and other structurally similar aromatic ketones and can be used in the synthesis of flavoring substances, since the ester formed by benzylacetone oxidation has a pronounced fruity smell Thermobifida fusca

Cloned(Commentary)

Cloned (Comment) Organism
gene pamO, recombinant expression of His-tagged enzyme in three different forms (C, N, and L) concerning the position of His-tag in Escherichia coli strain BL21(DE3), the His-tag locates at the N- and C-termini of the enzyme (PAMO N and PAMO C, respectively), PAMO L describes a construct with the His-tag distanced from the enzyme C-terminus with an additional 19-amino acid sequence, the enzyme forms do not show great differences in catalytic activity. No use of the arabinose promoter in the expression system Thermobifida fusca

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
phenylacetone + NADPH + H+ + O2 Thermobifida fusca
-
benzyl acetate + NADP+ + H2O
-
?

Organism

Organism UniProt Comment Textmining
Thermobifida fusca Q47PU3
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
additional information no posttranslational modification in the PAMO proteins expressed in Escherichia coli cells Thermobifida fusca

Purification (Commentary)

Purification (Comment) Organism
recombinant C-terminally His-tagged enzyme from Escherichia coli strain BL21(DE3) by one-step nickel affinity chromatography to near-homogeneity Thermobifida fusca

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phenylacetone + NADPH + H+ + O2
-
Thermobifida fusca benzyl acetate + NADP+ + H2O
-
?

Subunits

Subunits Comment Organism
monomer x * 62000, SDS-PAGE Thermobifida fusca

Synonyms

Synonyms Comment Organism
PAMO
-
Thermobifida fusca

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Thermobifida fusca

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Thermobifida fusca

Cofactor

Cofactor Comment Organism Structure
FAD
-
Thermobifida fusca
NADPH
-
Thermobifida fusca

General Information

General Information Comment Organism
physiological function phenylacetone monooxygenase (PAMO) catalyzes oxidation of ketones with molecular oxygen and NADPH with the formation of esters Thermobifida fusca