Protein Variants | Comment | Organism |
---|---|---|
C65D | site-directed mutagenesis, the engineered TfPAMO variant acts as an NADPH oxidase, it shows an extremely high rate of uncoupling compared with the wild-type enzyme. The mutant is not effective in stabilizing the C(4alpha)-peroxyflavin intermediate. For TfPAMO C65D, hydrogen peroxide and superoxide levels are highest at pH 9.0 | Thermobifida fusca |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | no detrimental effect of the accumulation of superoxide on biocatalysis is demonstrated | Thermobifida fusca |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
phenylacetone + NADPH + H+ + O2 | Thermobifida fusca | - |
benzyl acetate + NADP+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermobifida fusca | Q47PU3 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | for wild-type TfPAMO, the uncoupling side activity at different pHs ranges from 5.0 to 7.8%. Formation of hydrogen peroxide is rather constant (around 4.0 microM), while formation of superoxide is almost absent at low pH of 6.0, reaching 3.0 microM at high pH of 9.0-10.0 | Thermobifida fusca | ? | - |
- |
|
phenylacetone + NADPH + H+ + O2 | - |
Thermobifida fusca | benzyl acetate + NADP+ + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PAMO | - |
Thermobifida fusca |
Tf PAMO | - |
Thermobifida fusca |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Thermobifida fusca |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Thermobifida fusca |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 10 | activity range, profile overview | Thermobifida fusca |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Thermobifida fusca | |
NADPH | - |
Thermobifida fusca |
General Information | Comment | Organism |
---|---|---|
evolution | phenylacetone monooxygenase from Thermobifida fusca (TfPAMO) is a thermostable Baeyer-Villiger monooxygenase from the NAD(P)H/FAD-dependent oxidoreductase family | Thermobifida fusca |