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Literature summary for 1.14.13.92 extracted from

  • Carvalho, A.T.P.; Dourado, D.F.A.R.; Skvortsov, T.; de Abreu, M.; Ferguson, L.J.; Quinn, D.J.; Moody, T.S.; Huang, M.
    Catalytic mechanism of phenylacetone monooxygenases for non-native linear substrates (2017), Phys. Chem. Chem. Phys., 19, 26851-26861 .
    View publication on PubMed

Application

Application Comment Organism
synthesis phenylacetone monooxygenase (PAMO) is the most stable and thermo-tolerant member of the Baeyer-Villiger monooxygenase family, and the engineered enzyme is useful for the synthesis of industrially relevant compounds, in particular, in the biotransformation of long-chain aliphatic oils into potential biodiesels Thermobifida fusca

Protein Variants

Protein Variants Comment Organism
additional information rational engineering of enzyme PAMO for wide applications in industrial biocatalysis, in particular, in the biotransformation of long-chain aliphatic oils into potential biodiesels Thermobifida fusca

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information Michaelis-Menten kinetics Thermobifida fusca

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
phenylacetone + NADPH + H+ + O2 Thermobifida fusca
-
benzyl acetate + NADP+ + H2O
-
?

Organism

Organism UniProt Comment Textmining
Thermobifida fusca Q47PU3
-
-

Reaction

Reaction Comment Organism Reaction ID
phenylacetone + NADPH + H+ + O2 = benzyl acetate + NADP+ + H2O catalytic mechanism, via C4a-peroxyflavin and Criegee intermediates, of phenylacetone monooxygenases for the native substrate phenylacetone as well as for a linear non-native substrate 2-octanone, using molecular dynamics simulations, quantum mechanics and quantum mechanics/molecular mechanics calculations, and theoretical basis for the preference of the enzyme for the native aromatic substrate over non-native linear substrates, overview Thermobifida fusca

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-octanone + NADPH + H+ + O2
-
Thermobifida fusca ? + NADP+ + H2O
-
?
phenylacetone + NADPH + H+ + O2
-
Thermobifida fusca benzyl acetate + NADP+ + H2O
-
?

Synonyms

Synonyms Comment Organism
PAMO
-
Thermobifida fusca

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Thermobifida fusca

General Information

General Information Comment Organism
evolution phenylacetone monooxygenase is the most stable and thermo-tolerant member of the Baeyer-Villiger monooxygenase family Thermobifida fusca