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Literature summary for 1.14.13.9 extracted from

  • Hirai, K.; Kuroyanagi, H.; Tatebayashi, Y.; Hayashi, Y.; Hirabayashi-Takahashi, K.; Saito, K.; Haga, S.; Uemura, T.; Izumi, S.
    Dual role of the carboxyl-terminal region of pig liver L-kynurenine 3-monooxygenase: mitochondrial-targeting signal and enzymatic activity (2010), J. Biochem., 148, 639-650.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determnination and analysis, sequence comparisons, expression of FLAG-tagged wild-type enzyme and of C-terminal truncation mutants in COS-7 cells. The FLAG tag directs the wild-type enzyme to mitochondria but also to the cytosol and the plasma mambrane, overview Sus scrofa

Protein Variants

Protein Variants Comment Organism
additional information construction of C-terminal truncation mutants KMODELTAC10, KMODELTAC20, KMODELTAC30, KMODELTAC50, and KMODELTAC70, that are all localized in the cytosol after recombinant expression in COS-7 cells, exept for KMODELTAC10. Two forms of C-terminal truncation, KMODELTAC10D and KMODELTAC20D retain almost full activity but KMODELTAC30D shows 1.6fold higher activity than the wild-type. The activities of KMODELTAC50 and KMODELTAC70 is highly reduced, overview. The quantity of expressed KMODELTA30D in COS-7 cellsis 1.3fold higher than the wild-type KMO Sus scrofa

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrial outer membrane the C-terminal region functions as a mitochondrial targeting signal Sus scrofa 5741
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-kynurenine + NADH + H+ + O2 Sus scrofa
-
3-hydroxy-L-kynurenine + NAD+ + H2O
-
?
L-kynurenine + NADPH + H+ + O2 Sus scrofa
-
3-hydroxy-L-kynurenine + NADP+ + H2O
-
?

Organism

Organism UniProt Comment Textmining
Sus scrofa Q9MZS9
-
-

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Sus scrofa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-kynurenine + NADH + H+ + O2
-
Sus scrofa 3-hydroxy-L-kynurenine + NAD+ + H2O
-
?
L-kynurenine + NADPH + H+ + O2
-
Sus scrofa 3-hydroxy-L-kynurenine + NADP+ + H2O
-
?

Subunits

Subunits Comment Organism
More the C-terminal region is required for the enzymatic activity and functions as a mitochondrial targeting signal Sus scrofa

Synonyms

Synonyms Comment Organism
KMO
-
Sus scrofa
L-kynurenine 3-monooxygenase
-
Sus scrofa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Sus scrofa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Sus scrofa

Cofactor

Cofactor Comment Organism Structure
FAD
-
Sus scrofa
NADH
-
Sus scrofa
NADPH
-
Sus scrofa

General Information

General Information Comment Organism
additional information the C-terminal region of pig liver KMO plays a dual role. First, it is required for the enzymatic activity. Second, it functions as a mitochondrial targeting signal Sus scrofa