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Literature summary for 1.14.13.82 extracted from

  • Venkatesagowda, B.
    Enzymatic Kraft lignin demethylation and fungal O-demethylases like vanillate-O-demethylase and syringate O-demethylase catalyzed catechol-Fe3+ complexation method (2018), J. Microbiol. Methods, 152, 126-134 .
    View publication on PubMed

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
vanillate + O2 + NADH + H+ Aspergillus sp.
-
3,4-dihydroxybenzoate + NAD+ + H2O + formaldehyde
-
?
vanillate + O2 + NADH + H+ Galerina autumnalis
-
3,4-dihydroxybenzoate + NAD+ + H2O + formaldehyde
-
?

Organism

Organism UniProt Comment Textmining
Aspergillus sp.
-
-
-
Galerina autumnalis
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information to detect fungal demethylation and release of catechol-like structures, these are demonstrated using catechol, gallic acid and caffeic acid as standard model compounds to forms mono, bis- and/or tris-catechol-Fe3+ complexes. The catechol-Fe3+ complexes formation controlled by pH via the deprotonation of the catechol hydroxyls is investigated at pH 2.5, 8.0 and 10.0 and demonstrates that catechol formed mono, bis- and/or tris-catechol-Fe3+ complexes, and show maximum absorbance at 547 nm. Lignin demethylation (O-demethylase) and formation of dicatecholic structures is detected. The produced aromatic vicinal diol groups in lignin model compounds (LMCs) and KL are determined using different catecholic-binding reagents with the influence of H2O2, along with 4-antiaminopyrine reagent, and are analyzed by the following: 1. Fe3+-catechol complexation method, 2. HNO2 method, 3. FAS (ferric ammonium-sulfate) method, and 4. Ti(III)-NTA (titanium (III)-nitrilotriacetate) method for hydrolytic zone formation. Among the tested methods, Fe3+-catechol complexation shows lytic zone formation, mechanism, overview Aspergillus sp. ?
-
-
additional information to detect fungal demethylation and release of catechol-like structures, these are demonstrated using catechol, gallic acid and caffeic acid as standard model compounds to forms mono, bis- and/or tris-catechol-Fe3+ complexes. The catechol-Fe3+ complexes formation controlled by pH via the deprotonation of the catechol hydroxyls is investigated at pH 2.5, 8.0 and 10.0 and demonstrates that catechol formed mono, bis- and/or tris-catechol-Fe3+ complexes, and show maximum absorbance at 547 nm. Lignin demethylation (O-demethylase) and formation of pyrocatecholic structures is detected. The produced aromatic vicinal diol groups in lignin model compounds (LMCs) and KL are determined using different catecholic-binding reagents with the influence of H2O2, along with 4-antiaminopyrine reagent, and are analyzed by the following: 1. Fe3+-catechol complexation method, 2. HNO2 method, 3. FAS (ferric ammonium-sulfate) method, and 4. Ti(III)-NTA (titanium (III)-nitrilotriacetate) method for hydrolytic zone formation. Among the tested methods, Fe3+-catechol complexation shows lytic zone formation, mechanism, overview Galerina autumnalis ?
-
-
vanillate + O2 + NADH + H+
-
Aspergillus sp. 3,4-dihydroxybenzoate + NAD+ + H2O + formaldehyde
-
?
vanillate + O2 + NADH + H+
-
Galerina autumnalis 3,4-dihydroxybenzoate + NAD+ + H2O + formaldehyde
-
?

Synonyms

Synonyms Comment Organism
vanillate-O-demethylase
-
Aspergillus sp.
vanillate-O-demethylase
-
Galerina autumnalis

Cofactor

Cofactor Comment Organism Structure
NADH
-
Aspergillus sp.
NADH
-
Galerina autumnalis

General Information

General Information Comment Organism
metabolism lignin demethylation (O-demethylases) activity strips-off methyl groups in LMCs and KL and produces vicinal diols that covalently bind with Fe3+ to form Fe3+-catechol complexes Aspergillus sp.
metabolism lignin demethylation (O-demethylases) activity strips-off methyl groups in LMCs and KL and produces vicinal diols that covalently bind with Fe3+ to form Fe3+-catechol complexes Galerina autumnalis