Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
vanillate + O2 + NADH + H+ | Aspergillus sp. | - |
3,4-dihydroxybenzoate + NAD+ + H2O + formaldehyde | - |
? | |
vanillate + O2 + NADH + H+ | Galerina autumnalis | - |
3,4-dihydroxybenzoate + NAD+ + H2O + formaldehyde | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus sp. | - |
- |
- |
Galerina autumnalis | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | to detect fungal demethylation and release of catechol-like structures, these are demonstrated using catechol, gallic acid and caffeic acid as standard model compounds to forms mono, bis- and/or tris-catechol-Fe3+ complexes. The catechol-Fe3+ complexes formation controlled by pH via the deprotonation of the catechol hydroxyls is investigated at pH 2.5, 8.0 and 10.0 and demonstrates that catechol formed mono, bis- and/or tris-catechol-Fe3+ complexes, and show maximum absorbance at 547 nm. Lignin demethylation (O-demethylase) and formation of dicatecholic structures is detected. The produced aromatic vicinal diol groups in lignin model compounds (LMCs) and KL are determined using different catecholic-binding reagents with the influence of H2O2, along with 4-antiaminopyrine reagent, and are analyzed by the following: 1. Fe3+-catechol complexation method, 2. HNO2 method, 3. FAS (ferric ammonium-sulfate) method, and 4. Ti(III)-NTA (titanium (III)-nitrilotriacetate) method for hydrolytic zone formation. Among the tested methods, Fe3+-catechol complexation shows lytic zone formation, mechanism, overview | Aspergillus sp. | ? | - |
- |
|
additional information | to detect fungal demethylation and release of catechol-like structures, these are demonstrated using catechol, gallic acid and caffeic acid as standard model compounds to forms mono, bis- and/or tris-catechol-Fe3+ complexes. The catechol-Fe3+ complexes formation controlled by pH via the deprotonation of the catechol hydroxyls is investigated at pH 2.5, 8.0 and 10.0 and demonstrates that catechol formed mono, bis- and/or tris-catechol-Fe3+ complexes, and show maximum absorbance at 547 nm. Lignin demethylation (O-demethylase) and formation of pyrocatecholic structures is detected. The produced aromatic vicinal diol groups in lignin model compounds (LMCs) and KL are determined using different catecholic-binding reagents with the influence of H2O2, along with 4-antiaminopyrine reagent, and are analyzed by the following: 1. Fe3+-catechol complexation method, 2. HNO2 method, 3. FAS (ferric ammonium-sulfate) method, and 4. Ti(III)-NTA (titanium (III)-nitrilotriacetate) method for hydrolytic zone formation. Among the tested methods, Fe3+-catechol complexation shows lytic zone formation, mechanism, overview | Galerina autumnalis | ? | - |
- |
|
vanillate + O2 + NADH + H+ | - |
Aspergillus sp. | 3,4-dihydroxybenzoate + NAD+ + H2O + formaldehyde | - |
? | |
vanillate + O2 + NADH + H+ | - |
Galerina autumnalis | 3,4-dihydroxybenzoate + NAD+ + H2O + formaldehyde | - |
? |
Synonyms | Comment | Organism |
---|---|---|
vanillate-O-demethylase | - |
Aspergillus sp. |
vanillate-O-demethylase | - |
Galerina autumnalis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Aspergillus sp. | |
NADH | - |
Galerina autumnalis |
General Information | Comment | Organism |
---|---|---|
metabolism | lignin demethylation (O-demethylases) activity strips-off methyl groups in LMCs and KL and produces vicinal diols that covalently bind with Fe3+ to form Fe3+-catechol complexes | Aspergillus sp. |
metabolism | lignin demethylation (O-demethylases) activity strips-off methyl groups in LMCs and KL and produces vicinal diols that covalently bind with Fe3+ to form Fe3+-catechol complexes | Galerina autumnalis |