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Literature summary for 1.14.13.82 extracted from

  • Morawski, B.; Segura, A.; Ornston, L.N.
    Substrate range and genetic analysis of Acinetobacter vanillate demethylase (2000), J. Bacteriol., 182, 1383-1389.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Acinetobacter sp.

Protein Variants

Protein Variants Comment Organism
H156R substituting an iron-binding ligand Acinetobacter sp.
additional information several amino acid substitutions give indications of the extent to which amino acid substitutions can be tolerated at specified positions Acinetobacter sp.
W217R functional at 22øC but not at 37øC Acinetobacter sp.

Inhibitors

Inhibitors Comment Organism Structure
4-hydroxy-3,5-dimethylbenzoate 20% competitive inhibition at 3 mM Acinetobacter sp.
m-anisate 15-77.5% competitive inhibition of vanillate demethylation between 1-3 mM Acinetobacter sp.
m-toluate 45% competitive inhibition at 3 mM Acinetobacter sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
vanillate + O2 + NADH + H+ Acinetobacter sp. able to demethylate one methoxy group or to monohydroxylate one methyl group in the meta position 3,4-dihydroxybenzoate + NAD+ + H2O + formaldehyde
-
r

Organism

Organism UniProt Comment Textmining
Acinetobacter sp.
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strain ADP1 and ADP230
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Acinetobacter sp. ADP1
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strain ADP1 and ADP230
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Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
Acinetobacter sp.
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3,4,5-trimethoxybenzoate + O2 + NADH + H+ 70% of the activity compared to vanillate Acinetobacter sp. 3-hydroxy-4,5-dimethoxybenzoate + NAD+ + H2O + formaldehyde
-
?
3,4,5-trimethoxybenzoate + O2 + NADH + H+ 70% of the activity compared to vanillate Acinetobacter sp. ADP1 3-hydroxy-4,5-dimethoxybenzoate + NAD+ + H2O + formaldehyde
-
?
3,4-dimethoxybenzoate + O2 + NADH + H+
-
Acinetobacter sp. isovanillate + NAD+ + H2O + formaldehyde
-
?
3,4-dimethoxybenzoate + O2 + NADH + H+
-
Acinetobacter sp. ADP1 isovanillate + NAD+ + H2O + formaldehyde
-
?
3-(hydroxymethyl)-benzoate + NAD+ + H2O + formaldehyde
-
Acinetobacter sp. m-toluate + O2 + NADH + H+
-
?
3-(hydroxymethyl)-benzoate + NAD+ + H2O + formaldehyde
-
Acinetobacter sp. ADP1 m-toluate + O2 + NADH + H+
-
?
3-hydroxymethyl-4-hydroxy-5-methylbenzoate + NADH + H+ + ? 85% of the activity compared to vanillate Acinetobacter sp. 4-hydroxy-3,5-dimethylbenzoate + NAD+ + ?
-
?
3-hydroxymethyl-4-hydroxy-5-methylbenzoate + NADH + H+ + ? 85% of the activity compared to vanillate Acinetobacter sp. ADP1 4-hydroxy-3,5-dimethylbenzoate + NAD+ + ?
-
?
m-anisate + O2 + NADH + H+
-
Acinetobacter sp. m-hydroxybenzoate + NAD+ + H2O + formaldehyde
-
?
m-anisate + O2 + NADH + H+
-
Acinetobacter sp. ADP1 m-hydroxybenzoate + NAD+ + H2O + formaldehyde
-
?
vanillate + O2 + NADH + H+ able to demethylate one methoxy group or to monohydroxylate one methyl group in the meta position Acinetobacter sp. 3,4-dihydroxybenzoate + NAD+ + H2O + formaldehyde
-
r