Cloned (Comment) | Organism |
---|---|
gene Xantha-I, recombinant expression of active XanL strictly requires co-expression with an additional protein, Ycf54, recombinant expression of His-tagegd XanL and Ycf54 in Escherichia coli strain Artic Express (DE3) | Hordeum vulgare |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | assays performed with barley extract are inhibited by antibodies raised against ferredoxin and ferredoxin-NADPH oxidoreductase (FNR) | Hordeum vulgare |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast membrane | membrane-bound | Hordeum vulgare | 31969 | - |
etioplast | - |
Hordeum vulgare | 9513 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | required, the aerobic cyclase belongs to the family of diiron carboxylate-bridged proteins characterized by the iron-binding motif E-Xn-E-X-X-H-Xn-E-Xn-E-X-X-H | Hordeum vulgare | |
Mg2+ | required | Hordeum vulgare |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
magnesium-protoporphyrin IX 13-monomethyl ester + 3 NADPH + 3 H+ + 3 O2 | Hordeum vulgare | overall reaction | 3,8-divinyl protochlorophyllide a + 3 NADP+ + 5 H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hordeum vulgare | Q5EFU4 | cv. Bonus | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
magnesium-protoporphyrin IX 13-monomethyl ester + 3 NADPH + 3 H+ + 3 O2 = 3,8-divinyl protochlorophyllide a + 3 NADP+ + 5 H2O | overall reaction, reaction mechanism of the aerobic cyclase reaction, overview. The cyclase reaction is a six-electron redox reaction suggested to proceed via beta-hydroxy and beta-keto intermediates. The electrons are provided by Fd. NADPH can be used as the source of electrons transferred to ferredoxin (Fd) via ferredoxin-NADPH oxidoreductase (FNR). In green tissue, Fd can also be reduced by photosystem I | Hordeum vulgare |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
magnesium-protoporphyrin IX 13-monomethyl ester + 3 NADPH + 3 H+ + 3 O2 | overall reaction | Hordeum vulgare | 3,8-divinyl protochlorophyllide a + 3 NADP+ + 5 H2O | - |
? | |
additional information | in vitro cyclase activity is obtained with recombinant XanL in combination with ferredoxin (Fd) and ferredoxin-NADPH oxidoreductase (FNR). Fd and FNR are plastid-localized redox components. Enzymatic assays combining spinach Fd and FNR with XanL[coYcf54] show high activity | Hordeum vulgare | ? | - |
- |
Synonyms | Comment | Organism |
---|---|---|
CRD1 | - |
Hordeum vulgare |
Mg-protoporphyrin IX monomethyl ester cyclase | - |
Hordeum vulgare |
MPE cyclase | - |
Hordeum vulgare |
XanL | - |
Hordeum vulgare |
Xantha-l | - |
Hordeum vulgare |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Hordeum vulgare |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.1 | - |
assay at | Hordeum vulgare |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
Ferredoxin | the MPE cyclase is a ferredoxin-dependent enzyme | Hordeum vulgare | |
NADPH | - |
Hordeum vulgare |
General Information | Comment | Organism |
---|---|---|
evolution | two distinct enzymes have been identified that catalyze the cyclase reaction, originally distinguished by the source of the incorporated oxygen. The enzyme that catalyzes the cyclase reaction in the absence of molecular oxygen (anaerobic) derives the oxygen from water and is encoded by bchE in facultative photosynthetic bacteria like Rhodobacter sphaeroides. The anaerobic enzyme functions as a hydratase, whereas the aerobic cyclase is an oxygenase. The aerobic cyclase belongs to the family of diiron carboxylate-bridged proteins characterized by the iron-binding motif E-Xn-E-X-X-H-Xn-E-Xn-E-X-X-H. The cyclase activity requires both additional soluble and membrane-bound fractions | Hordeum vulgare |
metabolism | in the first unique step of the chlorophyll biosynthetic pathway, Mg2+ is inserted into protoporphyrin IX. Subsequently, a methyl group is transferred to the carboxyl group of the propionate on the C ring of Mg-protoporphyrin IX, generating Mg-protoporphyrin IX monomethyl ester (MPE), which is the substrate of the MPE cyclase. The cyclase catalyzes the formation of the isocyclic E ring by insertion of oxygen and attaching the methylated propionate to the methene bridge between pyrrole rings C and D, forming protochlorophyllide. Chlorophyll is obtained after additional reactions involving a light-dependent oxidation of protochlorophyllide to chlorophyllide, reduction of the vinyl group on the B ring, and, finally, addition of a polyisoprene tail | Hordeum vulgare |
physiological function | the Xantha-l gene product (XanL) is a membrane-bound diiron monooxygenase, which requires additional soluble and membrane-bound components for its activity. The enzyme XanL is a Mg-protoporphyrin IX monomethyl ester cyclase involved in the formation of the isocyclic E-ring characteristic of chlorophylls. The MPE cyclase is a ferredoxin-dependent enzyme. Ferredoxin is part of the photosynthetic electron-transport chain, which suggests that the cyclase reaction might be connected to photosynthesis under light conditions | Hordeum vulgare |