Cloned (Comment) | Organism |
---|---|
gene OsCRD1, encoding a putative subunit of enzyme MPEC, is a single-copy gene in rice, sequence comparisons, quantitative real-time RT-PCR enzyme expression analysis | Oryza sativa Japonica Group |
Protein Variants | Comment | Organism |
---|---|---|
A96T | site-directed mutagenesis, the point mutation in OsCRD1 does not change its location, but leads to deficiency in chlorophyll biosynthesis and chloroplast development and decreases photosynthetic capacity in rice, phenotype | Oryza sativa Japonica Group |
G286A | naturally occurring mutation, the mutation in enzyme MPEC causes the phenotype of the rice pale-green leaf mutant m167, phenotype | Oryza sativa Japonica Group |
additional information | mutant m167 is isolated from a EMS mutagenized population from the Japonica rice variety Kitaake. To construct the F2 mapping population, the yellow-green leaf rice mutant m167 is crossed with rice varieties Zhefu802 and Dular, respectively, genotyping of F2 population | Oryza sativa Japonica Group |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Oryza sativa Japonica Group | 9507 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
magnesium-protoporphyrin IX 13-monomethyl ester + 3 NADPH + 3 H+ + 3 O2 | Oryza sativa Japonica Group | - |
3,8-divinyl protochlorophyllide a + 3 NADP+ + 5 H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryza sativa Japonica Group | Q9SDJ2 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Oryza sativa Japonica Group | - |
leaf sheath | - |
Oryza sativa Japonica Group | - |
additional information | gene OsCRD1 is mainly expressed in green tissues, including stem, leaf, tassel and sheath, but not in root | Oryza sativa Japonica Group | - |
seedling | - |
Oryza sativa Japonica Group | - |
stem | - |
Oryza sativa Japonica Group | - |
tassel | - |
Oryza sativa Japonica Group | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
magnesium-protoporphyrin IX 13-monomethyl ester + 3 NADPH + 3 H+ + 3 O2 | - |
Oryza sativa Japonica Group | 3,8-divinyl protochlorophyllide a + 3 NADP+ + 5 H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
LOC_Os01g17170 | locus name | Oryza sativa Japonica Group |
Mg-protoporphyrin IX monomethyl ester cyclase | - |
Oryza sativa Japonica Group |
MPEC | - |
Oryza sativa Japonica Group |
OsCRD1 | - |
Oryza sativa Japonica Group |
rice copper response defect 1 | i.e. OsCRD1, a putative subunit of enzyme MPEC | Oryza sativa Japonica Group |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Oryza sativa Japonica Group |
General Information | Comment | Organism |
---|---|---|
malfunction | mutation of in enzyme MPEC causes the pale-green leaf mutant phenotype of mutant m167 with yellow-green leaves across the whole lifespan. Chlorophyll content decreases by 43-51% and the granal stacks of chloroplasts becomes thinner in m167. Chlorophyll fluorescence parameters, including Fv/Fm (the maximum quantum efficiency of PSII) and quantum yield of PSII (Y(II)), are lower in m167 than those in wild-type plants, and photosynthesis rate decreases by 40% in leaves of m167 mutant compared with wild-type plants, which leads to yield reduction in m167 | Oryza sativa Japonica Group |