Cloned (Comment) | Organism |
---|---|
gene ygl8 encodes a catalytic subunit of MgPME cyclase, DNA and amino acid sequence determination and analysis, map-based cloning, sequence comparisons and phylogenetic analysis. Transient expression in Nicotiana benthamiana. The recombinant construct pC1305-YGL8 is introduced into the ygl8 rice mutant by Agrobacterium tumefaciens EHA105-mediated transformation. Quantitative real-time PCR expression analysis | Oryza sativa |
Protein Variants | Comment | Organism |
---|---|---|
additional information | isolation of an Oryza sativa yellow-green leaf 8 (ygl8) mutant that exhibits chlorosis phenotype with abnormal chloroplast development in young leaves. During the development of leaves, the chlorotic plants turn green accompanied by restorations in chlorophyll content and chloroplast ultrastructure. Expression levels of some nuclear genes associated with Chl biosynthesis are affected in both the ygl8 mutant and YGL8 RNA interference lines. Phenotype, overview. Ygl8 knockout by RNAi | Oryza sativa |
N182S | the ygl8 mutation causes a conserved amino acid substitution, which is related to the alterations of chlorophyll precursor content | Oryza sativa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast membrane | the YGL8 subunit contains a chloroplast transit peptide | Oryza sativa | 31969 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
magnesium-protoporphyrin IX 13-monomethyl ester + 3 NADPH + 3 H+ + 3 O2 | Oryza sativa | - |
3,8-divinyl protochlorophyllide a + 3 NADP+ + 5 H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryza sativa | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | young, high enzyme content | Oryza sativa | - |
additional information | subunit YGL8 is constitutively expressed in various tissues, with more abundance in young leaves and panicles | Oryza sativa | - |
panicle | high enzyme content | Oryza sativa | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
magnesium-protoporphyrin IX 13-monomethyl ester + 3 NADPH + 3 H+ + 3 O2 | - |
Oryza sativa | 3,8-divinyl protochlorophyllide a + 3 NADP+ + 5 H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
magnesium-protoporphyrin IX monomethyl ester cyclase | - |
Oryza sativa |
MgPME cyclase | - |
Oryza sativa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Oryza sativa |
General Information | Comment | Organism |
---|---|---|
malfunction | Oryza sativa yellow-green leaf 8 (ygl8) mutant exhibits a chlorosis phenotype with abnormal chloroplast development in young leaves. During the development of leaves, the chlorotic plants turn green accompanied by restorations in chlorophyll content and chloroplast ultrastructure, expression levels of some nuclear genes associated with Chl biosynthesis are affected in both the ygl8 mutant and YGL8 RNA interference lines. YGL8 expression demonstrates no differences between the wild-type and ygl8 mutant plants, but the mutant shows increase of Proto IX and MgP/MgPME expression, accompanied with the decrease of protochlorophyllide. Phenotype, overview | Oryza sativa |
additional information | protein YGL8 has the dual functions in chlorophyll biosynthesis: one as a catalytic subunit of MgPME cyclase, the other as a core component of FLU-YGL8-LCAA-POR complex in chlorophyll biosynthesis, physical interaction between YGL8 and a rice chloroplast protein, low chlorophyll accumulation A (OsLCAA). YGL8 also interacts with the other two rice chloroplast proteins, viz. fluorescent (OsFLU1) and NADPH:protochlorophyllide oxidoreductase (OsPORB) | Oryza sativa |