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Literature summary for 1.14.13.81 extracted from

  • Liu, H.; Zheng, C.
    MPEC: An important gene in the chlorophyll biosynthesis pathway in photosynthetic organisms (2008), Photosynthetica, 46, 321-328.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
CN- iron chelator strongly inhibits enzyme in reconstituted in vitro assay but not or only slightly in intact chloroplast systems with membrane-bound haemoprotein Cucumis sativus
N3- iron chelator strongly inhibits enzyme in reconstituted in vitro assay but not or only slightly in intact chloroplast systems with membrane-bound haemoprotein Cucumis sativus

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast envelope
-
Arabidopsis thaliana 9941
-
chloroplast membrane
-
Hordeum vulgare 31969
-
chloroplast membrane
-
Chlamydomonas reinhardtii 31969
-
chloroplast membrane membrane bound cell extract from cucumbers Cucumis sativus 31969
-
cytoplasm soluble cell extract from cucumbers Cucumis sativus 5737
-
additional information recombination of chloroplast membrane and cytoplasm fractions is necessary to restore cucumber cyclase activity in vitro Cucumis sativus
-
-
soluble
-
Hordeum vulgare
-
-
thylakoid membrane
-
Arabidopsis thaliana 42651
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
soluble fraction, membrane bound component is more labile Cucumis sativus
43000
-
calculted from predicted 370 amino acid residue sequence, 1402 bp, PNZIP from Pharbitis nil Ipomoea nil

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
magnesium-protoporphyrin IX monomethyl ester + NADPH + H+ + O2 Nicotiana tabacum
-
protochlorophyllide a + NADP+ + H2O intermediates are 6-beta-hydroxy analogue and 6-beta-oxo analogue of protochlorophyllide a ?
magnesium-protoporphyrin IX monomethyl ester + NADPH + H+ + O2 Cucumis sativus
-
protochlorophyllide a + NADP+ + H2O intermediates are 6-beta-hydroxy analogue and 6-beta-oxo analogue of protochlorophyllide a ?
magnesium-protoporphyrin IX monomethyl ester + NADPH + H+ + O2 Oryza sativa
-
protochlorophyllide a + NADP+ + H2O intermediates are 6-beta-hydroxy analogue and 6-beta-oxo analogue of protochlorophyllide a ?
magnesium-protoporphyrin IX monomethyl ester + NADPH + H+ + O2 Hordeum vulgare
-
protochlorophyllide a + NADP+ + H2O intermediates are 6-beta-hydroxy analogue and 6-beta-oxo analogue of protochlorophyllide a ?
magnesium-protoporphyrin IX monomethyl ester + NADPH + H+ + O2 Rosa davurica
-
protochlorophyllide a + NADP+ + H2O intermediates are 6-beta-hydroxy analogue and 6-beta-oxo analogue of protochlorophyllide a ?
magnesium-protoporphyrin IX monomethyl ester + NADPH + H+ + O2 Salix babylonica
-
protochlorophyllide a + NADP+ + H2O intermediates are 6-beta-hydroxy analogue and 6-beta-oxo analogue of protochlorophyllide a ?
magnesium-protoporphyrin IX monomethyl ester + NADPH + H+ + O2 Spinacia oleracea
-
protochlorophyllide a + NADP+ + H2O intermediates are 6-beta-hydroxy analogue and 6-beta-oxo analogue of protochlorophyllide a ?
magnesium-protoporphyrin IX monomethyl ester + NADPH + H+ + O2 Triticum aestivum
-
protochlorophyllide a + NADP+ + H2O intermediates are 6-beta-hydroxy analogue and 6-beta-oxo analogue of protochlorophyllide a ?
magnesium-protoporphyrin IX monomethyl ester + NADPH + H+ + O2 Trifolium repens
-
protochlorophyllide a + NADP+ + H2O intermediates are 6-beta-hydroxy analogue and 6-beta-oxo analogue of protochlorophyllide a ?
magnesium-protoporphyrin IX monomethyl ester + NADPH + H+ + O2 Arabidopsis thaliana
-
protochlorophyllide a + NADP+ + H2O intermediates are 6-beta-hydroxy analogue and 6-beta-oxo analogue of protochlorophyllide a ?
magnesium-protoporphyrin IX monomethyl ester + NADPH + H+ + O2 Chlamydomonas reinhardtii
-
protochlorophyllide a + NADP+ + H2O intermediates are 6-beta-hydroxy analogue and 6-beta-oxo analogue of protochlorophyllide a ?
magnesium-protoporphyrin IX monomethyl ester + NADPH + H+ + O2 Rubrivivax gelatinosus
-
protochlorophyllide a + NADP+ + H2O intermediates are 6-beta-hydroxy analogue and 6-beta-oxo analogue of protochlorophyllide a ?
magnesium-protoporphyrin IX monomethyl ester + NADPH + H+ + O2 Ipomoea nil
-
protochlorophyllide a + NADP+ + H2O intermediates are 6-beta-hydroxy analogue and 6-beta-oxo analogue of protochlorophyllide a ?
magnesium-protoporphyrin IX monomethyl ester + NADPH + H+ + O2 Brassica napus
-
protochlorophyllide a + NADP+ + H2O intermediates are 6-beta-hydroxy analogue and 6-beta-oxo analogue of protochlorophyllide a ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q9M591
-
-
Brassica napus Q7XJI2
-
-
Chlamydomonas reinhardtii Q9AR22 green alga
-
Chlamydomonas reinhardtii Q9LD46 green alga
-
Cucumis sativus
-
cucumber
-
Hordeum vulgare Q5EFU4 barley
-
Ipomoea nil Q40093 first MPEC gene identification in cDNA library (PNZIP)
-
Nicotiana tabacum
-
tobacco
-
Oryza sativa
-
rice
-
Rosa davurica
-
-
-
Rubrivivax gelatinosus P0DJN9 purple bacterium
-
Salix babylonica Q7XJI4
-
-
Spinacia oleracea Q7XJI5 spinach
-
Trifolium repens Q7XJI1
-
-
Triticum aestivum Q7XJI6 wheat
-

Source Tissue

Source Tissue Comment Organism Textmining
cotyledon
-
Cucumis sativus
-
leaf strongest expression Nicotiana tabacum
-
leaf wheat Triticum aestivum
-
mesophyll specifically expressed in photosynthetic active cells Ipomoea nil
-
additional information in photosynthetic tissue, undetectable in non-photosynthetic tissues Cucumis sativus
-
additional information in photosynthetic tissue, undetectable in non-photosynthetic tissues Oryza sativa
-
additional information in photosynthetic tissue, undetectable in non-photosynthetic tissues Hordeum vulgare
-
additional information in photosynthetic tissue, undetectable in non-photosynthetic tissues Rosa davurica
-
additional information in photosynthetic tissue, undetectable in non-photosynthetic tissues Salix babylonica
-
additional information in photosynthetic tissue, undetectable in non-photosynthetic tissues Spinacia oleracea
-
additional information in photosynthetic tissue, undetectable in non-photosynthetic tissues Triticum aestivum
-
additional information in photosynthetic tissue, undetectable in non-photosynthetic tissues Trifolium repens
-
additional information in photosynthetic tissue, undetectable in non-photosynthetic tissues Arabidopsis thaliana
-
additional information in photosynthetic tissue, undetectable in non-photosynthetic tissues Chlamydomonas reinhardtii
-
additional information in photosynthetic tissue, undetectable in non-photosynthetic tissues Rubrivivax gelatinosus
-
additional information in photosynthetic tissue, undetectable in non-photosynthetic tissues Ipomoea nil
-
additional information in photosynthetic tissue, undetectable in non-photosynthetic tissues Brassica napus
-
additional information no expression in root and flower, expression in photosynthetic tissue, undetectable in non-photosynthetic tissues Nicotiana tabacum
-
stem weaker expression than in stem Nicotiana tabacum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
magnesium-protoporphyrin IX monomethyl ester + NADPH + H+ + O2
-
Nicotiana tabacum protochlorophyllide a + NADP+ + H2O intermediates are 6-beta-hydroxy analogue and 6-beta-oxo analogue of protochlorophyllide a ?
magnesium-protoporphyrin IX monomethyl ester + NADPH + H+ + O2
-
Cucumis sativus protochlorophyllide a + NADP+ + H2O intermediates are 6-beta-hydroxy analogue and 6-beta-oxo analogue of protochlorophyllide a ?
magnesium-protoporphyrin IX monomethyl ester + NADPH + H+ + O2
-
Oryza sativa protochlorophyllide a + NADP+ + H2O intermediates are 6-beta-hydroxy analogue and 6-beta-oxo analogue of protochlorophyllide a ?
magnesium-protoporphyrin IX monomethyl ester + NADPH + H+ + O2
-
Hordeum vulgare protochlorophyllide a + NADP+ + H2O intermediates are 6-beta-hydroxy analogue and 6-beta-oxo analogue of protochlorophyllide a ?
magnesium-protoporphyrin IX monomethyl ester + NADPH + H+ + O2
-
Rosa davurica protochlorophyllide a + NADP+ + H2O intermediates are 6-beta-hydroxy analogue and 6-beta-oxo analogue of protochlorophyllide a ?
magnesium-protoporphyrin IX monomethyl ester + NADPH + H+ + O2
-
Salix babylonica protochlorophyllide a + NADP+ + H2O intermediates are 6-beta-hydroxy analogue and 6-beta-oxo analogue of protochlorophyllide a ?
magnesium-protoporphyrin IX monomethyl ester + NADPH + H+ + O2
-
Spinacia oleracea protochlorophyllide a + NADP+ + H2O intermediates are 6-beta-hydroxy analogue and 6-beta-oxo analogue of protochlorophyllide a ?
magnesium-protoporphyrin IX monomethyl ester + NADPH + H+ + O2
-
Triticum aestivum protochlorophyllide a + NADP+ + H2O intermediates are 6-beta-hydroxy analogue and 6-beta-oxo analogue of protochlorophyllide a ?
magnesium-protoporphyrin IX monomethyl ester + NADPH + H+ + O2
-
Trifolium repens protochlorophyllide a + NADP+ + H2O intermediates are 6-beta-hydroxy analogue and 6-beta-oxo analogue of protochlorophyllide a ?
magnesium-protoporphyrin IX monomethyl ester + NADPH + H+ + O2
-
Arabidopsis thaliana protochlorophyllide a + NADP+ + H2O intermediates are 6-beta-hydroxy analogue and 6-beta-oxo analogue of protochlorophyllide a ?
magnesium-protoporphyrin IX monomethyl ester + NADPH + H+ + O2
-
Chlamydomonas reinhardtii protochlorophyllide a + NADP+ + H2O intermediates are 6-beta-hydroxy analogue and 6-beta-oxo analogue of protochlorophyllide a ?
magnesium-protoporphyrin IX monomethyl ester + NADPH + H+ + O2
-
Rubrivivax gelatinosus protochlorophyllide a + NADP+ + H2O intermediates are 6-beta-hydroxy analogue and 6-beta-oxo analogue of protochlorophyllide a ?
magnesium-protoporphyrin IX monomethyl ester + NADPH + H+ + O2
-
Ipomoea nil protochlorophyllide a + NADP+ + H2O intermediates are 6-beta-hydroxy analogue and 6-beta-oxo analogue of protochlorophyllide a ?
magnesium-protoporphyrin IX monomethyl ester + NADPH + H+ + O2
-
Brassica napus protochlorophyllide a + NADP+ + H2O intermediates are 6-beta-hydroxy analogue and 6-beta-oxo analogue of protochlorophyllide a ?

Subunits

Subunits Comment Organism
heterodimer 2 * ?, highly conserved leucin zipper domain and EXnEXRH motif (binding binuclear-iron cluster) Nicotiana tabacum
heterodimer 2 * ?, highly conserved leucin zipper domain and EXnEXRH motif (binding binuclear-iron cluster) Cucumis sativus
heterodimer 2 * ?, highly conserved leucin zipper domain and EXnEXRH motif (binding binuclear-iron cluster) Chlamydomonas reinhardtii
heterodimer 2 * ?, highly conserved leucin zipper domain and EXnEXRH motif (binding binuclear-iron cluster) Rubrivivax gelatinosus
heterodimer 2 * ?, highly conserved leucin zipper domain and EXnEXRH motif (binding binuclear-iron cluster) Ipomoea nil
heterodimer 2 * ?, highly conserved leucin zipper domain and EXnEXRH motif (binding binuclear-iron cluster) Brassica napus
heterodimer highly conserved leucin zipper domain and EXnEXRH motif (binding binuclear-iron cluster) Oryza sativa
heterodimer highly conserved leucin zipper domain and EXnEXRH motif (binding binuclear-iron cluster) Hordeum vulgare
heterodimer highly conserved leucin zipper domain and EXnEXRH motif (binding binuclear-iron cluster) Rosa davurica
heterodimer highly conserved leucin zipper domain and EXnEXRH motif (binding binuclear-iron cluster) Salix babylonica
heterodimer highly conserved leucin zipper domain and EXnEXRH motif (binding binuclear-iron cluster) Spinacia oleracea
heterodimer highly conserved leucin zipper domain and EXnEXRH motif (binding binuclear-iron cluster) Triticum aestivum
heterodimer highly conserved leucin zipper domain and EXnEXRH motif (binding binuclear-iron cluster) Trifolium repens
heterodimer highly conserved leucin zipper domain and EXnEXRH motif (binding binuclear-iron cluster) Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
AcsF protein previously named orf358 Rubrivivax gelatinosus
ATZIP protein
-
Arabidopsis thaliana
BNZIP protein
-
Brassica napus
CHL27 protein
-
Arabidopsis thaliana
CHL27 protein Arabidopsis thaliana Arabidopsis thaliana
CRD1
-
Chlamydomonas reinhardtii
Crd1 protein Chlamydomonas reinhardtii, first identified as gene in response to copper deficiency, one of two isozymes Chlamydomonas reinhardtii
CSZIP protein
-
Cucumis sativus
Cth1 protein copper target homolog 1, isoenzyme to Crd1 Chlamydomonas reinhardtii
magnesium-protoporphyrin IX monomethyl ester cyclase
-
Nicotiana tabacum
magnesium-protoporphyrin IX monomethyl ester cyclase
-
Cucumis sativus
magnesium-protoporphyrin IX monomethyl ester cyclase
-
Oryza sativa
magnesium-protoporphyrin IX monomethyl ester cyclase
-
Hordeum vulgare
magnesium-protoporphyrin IX monomethyl ester cyclase
-
Rosa davurica
magnesium-protoporphyrin IX monomethyl ester cyclase
-
Salix babylonica
magnesium-protoporphyrin IX monomethyl ester cyclase
-
Spinacia oleracea
magnesium-protoporphyrin IX monomethyl ester cyclase
-
Triticum aestivum
magnesium-protoporphyrin IX monomethyl ester cyclase
-
Trifolium repens
magnesium-protoporphyrin IX monomethyl ester cyclase
-
Arabidopsis thaliana
magnesium-protoporphyrin IX monomethyl ester cyclase
-
Chlamydomonas reinhardtii
magnesium-protoporphyrin IX monomethyl ester cyclase
-
Rubrivivax gelatinosus
magnesium-protoporphyrin IX monomethyl ester cyclase
-
Ipomoea nil
magnesium-protoporphyrin IX monomethyl ester cyclase
-
Brassica napus
MgPME cyclase
-
Nicotiana tabacum
MgPME cyclase
-
Cucumis sativus
MgPME cyclase
-
Oryza sativa
MgPME cyclase
-
Hordeum vulgare
MgPME cyclase
-
Rosa davurica
MgPME cyclase
-
Salix babylonica
MgPME cyclase
-
Spinacia oleracea
MgPME cyclase
-
Triticum aestivum
MgPME cyclase
-
Trifolium repens
MgPME cyclase
-
Arabidopsis thaliana
MgPME cyclase
-
Chlamydomonas reinhardtii
MgPME cyclase
-
Rubrivivax gelatinosus
MgPME cyclase
-
Ipomoea nil
MgPME cyclase
-
Brassica napus
MPEC gene product
-
Nicotiana tabacum
MPEC gene product
-
Cucumis sativus
MPEC gene product
-
Oryza sativa
MPEC gene product
-
Hordeum vulgare
MPEC gene product
-
Rosa davurica
MPEC gene product
-
Salix babylonica
MPEC gene product
-
Spinacia oleracea
MPEC gene product
-
Triticum aestivum
MPEC gene product
-
Trifolium repens
MPEC gene product
-
Arabidopsis thaliana
MPEC gene product
-
Chlamydomonas reinhardtii
MPEC gene product
-
Rubrivivax gelatinosus
MPEC gene product
-
Ipomoea nil
MPEC gene product
-
Brassica napus
NTZIP protein
-
Nicotiana tabacum
OSZIP protein
-
Oryza sativa
PNZIP protein
-
Ipomoea nil
RDZIP protein
-
Rosa davurica
SBZIP protein
-
Salix babylonica
SOZIP protein
-
Spinacia oleracea
TAZIP protein
-
Triticum aestivum
TRZIP protein
-
Trifolium repens
Xantha-l
-
Hordeum vulgare

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Nicotiana tabacum
NADPH
-
Cucumis sativus
NADPH
-
Oryza sativa
NADPH
-
Hordeum vulgare
NADPH
-
Rosa davurica
NADPH
-
Salix babylonica
NADPH
-
Spinacia oleracea
NADPH
-
Triticum aestivum
NADPH
-
Trifolium repens
NADPH
-
Arabidopsis thaliana
NADPH
-
Chlamydomonas reinhardtii
NADPH
-
Rubrivivax gelatinosus
NADPH
-
Ipomoea nil
NADPH
-
Brassica napus

Expression

Organism Comment Expression
Ipomoea nil exposure to far-red radiation at the end of day or red radiation in the middle of night reduce PNZIP mRNA (phytochrome regulation in the dark) down
Nicotiana tabacum strong irradiance down
Ipomoea nil endogenous 24 h rhythm and regulation by phytochrome in the dark additional information
Nicotiana tabacum radiant energy and low temperature up

General Information

General Information Comment Organism
malfunction a Chl27-antisense Arabidopsis mutant accumulates the cyclase substrate magnesium-protoporphyrin IX monomethyl ester and does not produce protochlorophyllide a Arabidopsis thaliana
malfunction a Rubrivivax gelatinosus strain with disrupted acsF gene cannot synthesize bacteriochlorophyll under oxygenated conditions but accumulates the substrate magnesium-protoporphyrin IX monomethyl ester, in contrast, under low-oxygene conditions the phenotype is similar to the wild-type, an alternative pathway for the reaction exists vie the bchE genes Rubrivivax gelatinosus
malfunction crd1 strains fail to accumulate photosystem 1 (PS 1) and light-harvesting complex 1 (LHC 1) during hypoxia or copper deficiency, and have reduced amounts of LHC 2, Crd1 abundance is increased in copper or oxygen deficient cells Chlamydomonas reinhardtii
malfunction Cth1 accumulates in copper-sufficient, oxygenated cells Chlamydomonas reinhardtii
malfunction transgenic tobacco with antisense NTZIP-RNA displays chlorosis and a lack of the ability to turn green under normal growth conditions Nicotiana tabacum
malfunction xantha-l35 and viridis-k deficient mutants grown in the dark, fed with delta-aminolevulinic acid, accumulate the substrate magnesium-protoporphyrin IX monomethyl ester and produce reduced amounts of protochlorophyllide a, in vitro complementation assay with mutants the aerobic cyclase is composed of at least 3 gene products, 1 soluble and 2 membrane-bound proteins (xantha-l and viridian-k) Hordeum vulgare
metabolism protochlorophyllide a production (formation of the isocyclic ring) via several intermediates in the chlorophyll biosynthesis pathway Nicotiana tabacum
metabolism protochlorophyllide a production (formation of the isocyclic ring) via several intermediates in the chlorophyll biosynthesis pathway Cucumis sativus
metabolism protochlorophyllide a production (formation of the isocyclic ring) via several intermediates in the chlorophyll biosynthesis pathway Oryza sativa
metabolism protochlorophyllide a production (formation of the isocyclic ring) via several intermediates in the chlorophyll biosynthesis pathway Hordeum vulgare
metabolism protochlorophyllide a production (formation of the isocyclic ring) via several intermediates in the chlorophyll biosynthesis pathway Rosa davurica
metabolism protochlorophyllide a production (formation of the isocyclic ring) via several intermediates in the chlorophyll biosynthesis pathway Salix babylonica
metabolism protochlorophyllide a production (formation of the isocyclic ring) via several intermediates in the chlorophyll biosynthesis pathway Spinacia oleracea
metabolism protochlorophyllide a production (formation of the isocyclic ring) via several intermediates in the chlorophyll biosynthesis pathway Triticum aestivum
metabolism protochlorophyllide a production (formation of the isocyclic ring) via several intermediates in the chlorophyll biosynthesis pathway Trifolium repens
metabolism protochlorophyllide a production (formation of the isocyclic ring) via several intermediates in the chlorophyll biosynthesis pathway Arabidopsis thaliana
metabolism protochlorophyllide a production (formation of the isocyclic ring) via several intermediates in the chlorophyll biosynthesis pathway Chlamydomonas reinhardtii
metabolism protochlorophyllide a production (formation of the isocyclic ring) via several intermediates in the chlorophyll biosynthesis pathway Rubrivivax gelatinosus
metabolism protochlorophyllide a production (formation of the isocyclic ring) via several intermediates in the chlorophyll biosynthesis pathway Ipomoea nil
metabolism protochlorophyllide a production (formation of the isocyclic ring) via several intermediates in the chlorophyll biosynthesis pathway Brassica napus