Cloned (Comment) | Organism |
---|---|
RNA is isolated to synthesize cDNA and quantitative real-time PCR is carried out for anaerobic and semiaerobic growth conditions | Chloroflexus aurantiacus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | significant portion of the protein is not accessible to proteases, chlorosome envelop is a special lipid monolayer, complement of chlorophyll pigments, self-assembled into antenna structures | Chloroflexus aurantiacus | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
SDS-PAGE, trypsin-digested | Chloroflexus aurantiacus |
45000 | - |
calculated from the acsF gene sequence encoding 375 amino acid residues | Chloroflexus aurantiacus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
magnesium-protoporphyrin monomethyl ester + NADPH + H+ + O2 | Chloroflexus aurantiacus | bacteria are grown under anaerobic (dark green culture color) or semiaerobic (orange-red culture color) conditions and express the enzyme genes under both conditions to similar levels, though the enzyme is normally working under aerobic conditions | magnesium-divinyl-protochlorophyllide a + NADP+ + H2O | - |
? | |
magnesium-protoporphyrin monomethyl ester + NADPH + H+ + O2 | Chloroflexus aurantiacus J-10-fl | bacteria are grown under anaerobic (dark green culture color) or semiaerobic (orange-red culture color) conditions and express the enzyme genes under both conditions to similar levels, though the enzyme is normally working under aerobic conditions | magnesium-divinyl-protochlorophyllide a + NADP+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chloroflexus aurantiacus | A9WIR9 | green phototrophic bacterium | - |
Chloroflexus aurantiacus J-10-fl | A9WIR9 | green phototrophic bacterium | - |
Purification (Comment) | Organism |
---|---|
cells are centrifuged, sonicated, cell debris removed by centrifugation, membrane fraction separated from the soluble fraction by ultracentrifugation, chlorosome fractioned by sucrose density gradient (15-45%), for SDS-PAGE chlorosome fraction is incubated in methanol, centrifuged, the pellet containing the chlorosome envelop is subjected to SDS-PAGE, protein identification by MALDI-TOF and liquid chromatography-tandem mass spectrometry | Chloroflexus aurantiacus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
magnesium-protoporphyrin monomethyl ester + NADPH + H+ + O2 | bacteria are grown under anaerobic (dark green culture color) or semiaerobic (orange-red culture color) conditions and express the enzyme genes under both conditions to similar levels, though the enzyme is normally working under aerobic conditions | Chloroflexus aurantiacus | magnesium-divinyl-protochlorophyllide a + NADP+ + H2O | - |
? | |
magnesium-protoporphyrin monomethyl ester + NADPH + H+ + O2 | bacteria are grown under anaerobic (dark green culture color) or semiaerobic (orange-red culture color) conditions and express the enzyme genes under both conditions to similar levels, though the enzyme is normally working under aerobic conditions | Chloroflexus aurantiacus J-10-fl | magnesium-divinyl-protochlorophyllide a + NADP+ + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AcsF protein | - |
Chloroflexus aurantiacus |
magnesium-protoporphyrin IX monomethyl ester (oxidative) cyclase | - |
Chloroflexus aurantiacus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | high probability for binuclear iron motif is proposed by sequence alignment, and a significant iron amount in the chlorosome fraction cannot be assigned to other chlorosome proteins | Chloroflexus aurantiacus |
Organism | Comment | Expression |
---|---|---|
Chloroflexus aurantiacus | the aerobic magnesium-protoporphyrin IX monomethyl ester (oxidative) cyclase is expressed under semiaerobic and anaerobic conditions, the levels are similar under semiaerobic and anaerobic conditions | additional information |