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Literature summary for 1.14.13.8 extracted from

  • Catucci, G.; Gao, C.; Rampolla, G.; Gilardi, G.; Sadeghi, S.J.
    Uncoupled human flavin-containing monooxygenase 3 releases superoxide radical in addition to hydrogen peroxide (2019), Free Radic. Biol. Med., 145, 250-255 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene FMO3, recombinant expression of C-terminally His-tagged wild-type and mutant enzymes in Escherichia coli Homo sapiens

Protein Variants

Protein Variants Comment Organism
N61S an active site polymorphic variant, the single-nucleotide polymorphism is leading to deleterious effects of oxidative stress. N-Oxygenation of benzydamine by the wild-type and N61S mutant variant of FMO3. In the absence of the substrate benzydamine (BZD), N61S produces higher amounts of H2O2 compared to wild-type Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum
-
Homo sapiens 5783
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
benzydamine + NADPH + H+ + O2 Homo sapiens
-
benzydamine N-oxide + NADP+ + H2O
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens P31513
-
-

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzydamine + NADPH + H+ + O2
-
Homo sapiens benzydamine N-oxide + NADP+ + H2O
-
?
benzydamine + NADPH + H+ + O2 N-oxygenation of benzydamine by the wild-type and N61S mutant variant of FMO3 Homo sapiens benzydamine N-oxide + NADP+ + H2O
-
?
additional information analysis of the uncoupling reactions in the catalytic cycle of enzyme FMO3, overview. The level of uncoupling varies between 50% and 70% (wild-type) and 90-98% (mutant N61S) for incubations with NADPH and benzydamine over a period of 5 or 20 min, respectively. The substrate lowers the level of uncoupling only related to the H2O2 and not the superoxide radical. In the absence of the substrate benzydamine (BZD), mutant N61S produces higher amounts of H2O2 compared to wild-type Homo sapiens ?
-
-

Synonyms

Synonyms Comment Organism
flavin-containing monooxygenase 3
-
Homo sapiens
FMO3
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Homo sapiens
hFMO3
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
FAD
-
Homo sapiens
NADPH
-
Homo sapiens

General Information

General Information Comment Organism
physiological function human flavin-containing monooxygenase 3 (hFMO3) is a drug-metabolizing enzyme capable of performing N- or S-oxidation using the C4a-hydroperoxy intermediate. Importance of the involvement of hFMO3 in the production of radicals in the endoplasmic reticulum Homo sapiens