Cloned (Comment) | Organism |
---|---|
gene FMO3, recombinant expression of C-terminally His-tagged wild-type and mutant enzymes in Escherichia coli | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
N61S | an active site polymorphic variant, the single-nucleotide polymorphism is leading to deleterious effects of oxidative stress. N-Oxygenation of benzydamine by the wild-type and N61S mutant variant of FMO3. In the absence of the substrate benzydamine (BZD), N61S produces higher amounts of H2O2 compared to wild-type | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endoplasmic reticulum | - |
Homo sapiens | 5783 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzydamine + NADPH + H+ + O2 | Homo sapiens | - |
benzydamine N-oxide + NADP+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P31513 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzydamine + NADPH + H+ + O2 | - |
Homo sapiens | benzydamine N-oxide + NADP+ + H2O | - |
? | |
benzydamine + NADPH + H+ + O2 | N-oxygenation of benzydamine by the wild-type and N61S mutant variant of FMO3 | Homo sapiens | benzydamine N-oxide + NADP+ + H2O | - |
? | |
additional information | analysis of the uncoupling reactions in the catalytic cycle of enzyme FMO3, overview. The level of uncoupling varies between 50% and 70% (wild-type) and 90-98% (mutant N61S) for incubations with NADPH and benzydamine over a period of 5 or 20 min, respectively. The substrate lowers the level of uncoupling only related to the H2O2 and not the superoxide radical. In the absence of the substrate benzydamine (BZD), mutant N61S produces higher amounts of H2O2 compared to wild-type | Homo sapiens | ? | - |
- |
Synonyms | Comment | Organism |
---|---|---|
flavin-containing monooxygenase 3 | - |
Homo sapiens |
FMO3 | - |
Homo sapiens |
hFMO3 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Homo sapiens | |
NADPH | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
physiological function | human flavin-containing monooxygenase 3 (hFMO3) is a drug-metabolizing enzyme capable of performing N- or S-oxidation using the C4a-hydroperoxy intermediate. Importance of the involvement of hFMO3 in the production of radicals in the endoplasmic reticulum | Homo sapiens |