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Literature summary for 1.14.13.7 extracted from

  • Cafaro, V.; Notomista, E.; Capasso, P.; Di Donato, A.
    Mutation of glutamic acid 103 of toluene o-xylene monooxygenase as a means to control the catabolic efficiency of a recombinant upper pathway for degradation of methylated aromatic compounds (2005), Appl. Environ. Microbiol., 71, 4744-4750.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.13.7

Cloned(Commentary)

Cloned (Comment) Organism
co-expression with wild-type or E103 mutant toluene o-xylene monooxygenases in Escherichia coli strain JM109 Pseudomonas stutzeri

Protein Variants

Protein Variants Comment Organism
additional information production ToMO mutants with modified regioselectivity compared with the regioselectivity of the wild-type protein in order to alter the ability of the recombinant upper pathway to produce methylcatechol isomers from toluene and to produce 3,4-dimethylcatechol from o-xylene, the combination of ToMO mutant E103G and phenol oxidase increases the production of 4-methylcatechol from toluene and the formation of 3,4-dimethylcatechol from o-xylene, overview Pseudomonas stutzeri

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 2-xylene + 2 NADPH + 2 H+ + 2 O2 Pseudomonas stutzeri
-
 2,3-dimethylphenol + 3,4-dimethylphenol + 2 NADP+ + 2 H2O
-
 ?
2 2-xylene + 2 NADPH + 2 H+ + 2 O2 Pseudomonas stutzeri OX1
-
 2,3-dimethylphenol + 3,4-dimethylphenol + 2 NADP+ + 2 H2O
-
 ?
3 toluene + 3 NADPH + 3 H+ + 3 O2 Pseudomonas stutzeri
-
 2-cresol + 3-cresol + 4-cresol + 3 NADP+ + 3 H2O
-
 ?
3 toluene + 3 NADPH + 3 H+ + 3 O2 Pseudomonas stutzeri OX1
-
 2-cresol + 3-cresol + 4-cresol + 3 NADP+ + 3 H2O
-
 ?
phenol + NADPH + O2 Pseudomonas stutzeri
-
 catechol + NADP+ + H2O
-
 ?
phenol + NADPH + O2 Pseudomonas stutzeri OX1
-
 catechol + NADP+ + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas stutzeri
-
-
-
Pseudomonas stutzeri OX1
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 2-xylene + 2 NADPH + 2 H+ + 2 O2
-
 Pseudomonas stutzeri 2,3-dimethylphenol + 3,4-dimethylphenol + 2 NADP+ + 2 H2O
-
 ?
2 2-xylene + 2 NADPH + 2 H+ + 2 O2
-
 Pseudomonas stutzeri OX1 2,3-dimethylphenol + 3,4-dimethylphenol + 2 NADP+ + 2 H2O
-
 ?
3 toluene + 3 NADPH + 3 H+ + 3 O2
-
 Pseudomonas stutzeri 2-cresol + 3-cresol + 4-cresol + 3 NADP+ + 3 H2O
-
 ?
3 toluene + 3 NADPH + 3 H+ + 3 O2
-
 Pseudomonas stutzeri OX1 2-cresol + 3-cresol + 4-cresol + 3 NADP+ + 3 H2O
-
 ?
phenol + NADPH + O2
-
 Pseudomonas stutzeri catechol + NADP+ + H2O
-
 ?
phenol + NADPH + O2
-
 Pseudomonas stutzeri OX1 catechol + NADP+ + H2O
-
 ?

Synonyms

Synonyms Comment Organism
phenol hydroxylase
-
 Pseudomonas stutzeri

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Pseudomonas stutzeri