KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03779 | - |
NADH | phenol hydrolxylase component P | Pseudomonas stutzeri | |
0.61 | - |
phenol | reconstituted enzyme complex | Pseudomonas stutzeri | |
0.9 | - |
NADPH | phenol hydrolxylase component P | Pseudomonas stutzeri |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
phenol + NADH + H+ + O2 | Pseudomonas stutzeri | coupling between phenol hydroxylase and toluene/o-xylene monooxygenase optimizes the use of nonhydroxylated aromatic molecules by the draining effect of phenol hydroxylase on the products of oxidation catalyzed by toluene/o-xylene monooxygenase, thus avoiding phenol accumulation | catechol + NAD+ + H2O | - |
? | |
phenol + NADH + H+ + O2 | Pseudomonas stutzeri OX1 | coupling between phenol hydroxylase and toluene/o-xylene monooxygenase optimizes the use of nonhydroxylated aromatic molecules by the draining effect of phenol hydroxylase on the products of oxidation catalyzed by toluene/o-xylene monooxygenase, thus avoiding phenol accumulation | catechol + NAD+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas stutzeri | - |
- |
- |
Pseudomonas stutzeri OX1 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phenol + NADH + H+ + O2 | - |
Pseudomonas stutzeri | catechol + NAD+ + H2O | - |
? | |
phenol + NADH + H+ + O2 | coupling between phenol hydroxylase and toluene/o-xylene monooxygenase optimizes the use of nonhydroxylated aromatic molecules by the draining effect of phenol hydroxylase on the products of oxidation catalyzed by toluene/o-xylene monooxygenase, thus avoiding phenol accumulation | Pseudomonas stutzeri | catechol + NAD+ + H2O | - |
? | |
phenol + NADH + H+ + O2 | - |
Pseudomonas stutzeri OX1 | catechol + NAD+ + H2O | - |
? | |
phenol + NADH + H+ + O2 | coupling between phenol hydroxylase and toluene/o-xylene monooxygenase optimizes the use of nonhydroxylated aromatic molecules by the draining effect of phenol hydroxylase on the products of oxidation catalyzed by toluene/o-xylene monooxygenase, thus avoiding phenol accumulation | Pseudomonas stutzeri OX1 | catechol + NAD+ + H2O | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.286 | - |
phenol | reconstituted enzyme complex | Pseudomonas stutzeri |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | the phenol hydrolxylase component P reduces several artificial electron acceptors such as horse heart cytochrome c, 2,6-dichlorophenolindophenol, and potassium ferricyanide, with either NADH or NADPH as electron donor. NADH is preferentially used | Pseudomonas stutzeri | |
NADPH | the phenol hydrolxylase component P reduces several artificial electron acceptors such as horse heart cytochrome c, 2,6-dichlorophenolindophenol, and potassium ferricyanide, with either NADH or NADPH as electron donor. NADH is preferentially used | Pseudomonas stutzeri |