Cloned (Comment) | Organism |
---|---|
yes, gene mnx1, quantitative real-time PCR enzyme expression analysis, recombinant overexpression in Yarrowia lipolytica strain polf, coexpression with chorismate pyruvate-lyase (UbiC) and hydroquinone glucosyltransferase (AS) | Yarrowia lipolytica |
Protein Variants | Comment | Organism |
---|---|---|
additional information | Yarrowia lipolytica is engineered for enhanced production of arbutin. To construct the arbutin synthetic pathway in Yarrowia lipolytica, three genes, chorismate pyruvate-lyase (UbiC), 4-hydroxybenzoate 1-hydroxylase (MNX1), and hydroquinone glucosyltransferase (AS), are codon-optimized and heterologously expressed in Yarrowia lipolytica strain polf (leucine and uracil auxotroph). To maximize arbutin production, seven arbutin-biosynthesis molecular targets are overexpressed, and individual strengthening of DHS1 and DHS2 leads to an 8.9 and 7.8fold improvement in arbutin yield, respectively. Through optimization, a maximum arbutin titer of 8.6 g/l is achieved using the finally engineered Yarrowia lipolytica strain po1f-At0 Improvement in arbutin yields by glucose feeding | Yarrowia lipolytica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-hydroxybenzoate + NAD(P)H + 2 H+ + O2 | Yarrowia lipolytica | - |
hydroquinone + NAD(P)+ + H2O + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Yarrowia lipolytica | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-hydroxybenzoate + NAD(P)H + 2 H+ + O2 | - |
Yarrowia lipolytica | hydroquinone + NAD(P)+ + H2O + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MNX1 | - |
Yarrowia lipolytica |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Yarrowia lipolytica | |
NAD(P)H | - |
Yarrowia lipolytica |