KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | analysis of steady-state and rapid reaction conditions using primary and solvent kinetic isotope effects, stopped-flow and steady-state kinetics, overview | Mycolicibacterium smegmatis | |
2.4 | - |
L-lysine | pH 7.5, 25°C, with NADH | Mycolicibacterium smegmatis | |
12 | - |
L-lysine | pH 7.5, 25°C, with NADPH | Mycolicibacterium smegmatis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-lysine + NADH + H+ + O2 | Mycolicibacterium smegmatis | - |
N6-hydroxy-L-lysine + NAD+ + H2O | - |
? | |
L-lysine + NADH + H+ + O2 | Mycolicibacterium smegmatis G | - |
N6-hydroxy-L-lysine + NAD+ + H2O | - |
? | |
L-lysine + NADPH + H+ + O2 | Mycolicibacterium smegmatis | - |
N6-hydroxy-L-lysine + NADP+ + H2O | - |
? | |
L-lysine + NADPH + H+ + O2 | Mycolicibacterium smegmatis G | - |
N6-hydroxy-L-lysine + NADP+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycolicibacterium smegmatis | - |
- |
- |
Mycolicibacterium smegmatis G | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
L-lysine + NADPH + H+ + O2 = N6-hydroxy-L-lysine + NADP+ + H2O | unique mechanism where a rate-limiting and pH-sensitive conformational change occurs in the reductive half-reaction, which affects the efficiency of lysine hydroxylation | Mycolicibacterium smegmatis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-lysine + NADH + H+ + O2 | - |
Mycolicibacterium smegmatis | N6-hydroxy-L-lysine + NAD+ + H2O | - |
? | |
L-lysine + NADH + H+ + O2 | the hydride transfer is rate-limiting | Mycolicibacterium smegmatis | N6-hydroxy-L-lysine + NAD+ + H2O | - |
? | |
L-lysine + NADH + H+ + O2 | - |
Mycolicibacterium smegmatis G | N6-hydroxy-L-lysine + NAD+ + H2O | - |
? | |
L-lysine + NADH + H+ + O2 | the hydride transfer is rate-limiting | Mycolicibacterium smegmatis G | N6-hydroxy-L-lysine + NAD+ + H2O | - |
? | |
L-lysine + NADPH + H+ + O2 | - |
Mycolicibacterium smegmatis | N6-hydroxy-L-lysine + NADP+ + H2O | - |
? | |
L-lysine + NADPH + H+ + O2 | the hydride transfer is rate-limiting | Mycolicibacterium smegmatis | N6-hydroxy-L-lysine + NADP+ + H2O | - |
? | |
L-lysine + NADPH + H+ + O2 | - |
Mycolicibacterium smegmatis G | N6-hydroxy-L-lysine + NADP+ + H2O | - |
? | |
L-lysine + NADPH + H+ + O2 | the hydride transfer is rate-limiting | Mycolicibacterium smegmatis G | N6-hydroxy-L-lysine + NADP+ + H2O | - |
? | |
additional information | MbsG is non-specific for reduced pyridine dinucleotide, as it can utilize both NADH and NADPH with similar catalytic efficiency. But MbsG is specific for L-lysine and shows no activity with L-ornithine, 6-amino-1-hexanol, L-arginine, putrescine, and cadaverine | Mycolicibacterium smegmatis | ? | - |
? | |
additional information | MbsG is non-specific for reduced pyridine dinucleotide, as it can utilize both NADH and NADPH with similar catalytic efficiency. But MbsG is specific for L-lysine and shows no activity with L-ornithine, 6-amino-1-hexanol, L-arginine, putrescine, and cadaverine | Mycolicibacterium smegmatis G | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
flavin-dependent N6-lysine monooxygenase | - |
Mycolicibacterium smegmatis |
MbsG | - |
Mycolicibacterium smegmatis |
N-hydroxylating monooxygenase | - |
Mycolicibacterium smegmatis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Mycolicibacterium smegmatis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.43 | - |
L-lysine | pH 7.5, 25°C, with NADH | Mycolicibacterium smegmatis | |
0.55 | - |
L-lysine | pH 7.5, 25°C, with NADPH | Mycolicibacterium smegmatis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Mycolicibacterium smegmatis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.4 | 9 | stable higher activity within this range | Mycolicibacterium smegmatis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | dependent on | Mycolicibacterium smegmatis | |
additional information | activity measurements with isotopes of NAD(P)H, kinetic isotope effects, overview | Mycolicibacterium smegmatis | |
NADH | - |
Mycolicibacterium smegmatis | |
NADPH | - |
Mycolicibacterium smegmatis |
General Information | Comment | Organism |
---|---|---|
physiological function | Mycobacterium smegmatis G (MbsG) is a flavin-dependent L-lysine monooxygenase involved in the biosynthesis of the siderophore mycobactin, overview | Mycolicibacterium smegmatis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.046 | - |
L-lysine | pH 7.5, 25°C, with NADPH | Mycolicibacterium smegmatis | |
0.179 | - |
L-lysine | pH 7.5, 25°C, with NADH | Mycolicibacterium smegmatis |