Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
pentachlorophenol + NADPH + H+ + O2 | Sphingobium chlorophenolicum | degradation of pentachlorophenol | 2,3,5,6-tetrachloro-1,4-benzoquinone + NADP+ + chloride + H2O | - |
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Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sphingobium chlorophenolicum | P42535 | - |
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Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pentachlorophenol + NADPH + H+ + O2 | degradation of pentachlorophenol | Sphingobium chlorophenolicum | 2,3,5,6-tetrachloro-1,4-benzoquinone + NADP+ + chloride + H2O | - |
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pentachlorophenol + NADPH + H+ + O2 | the enzyme is an unusually slow flavin-dependent monooxygenase (kcat = 0.02/s) that converts pentachlorophenol to a highly reactive product, tetrachlorobenzoquinone. By stopped-flow spectroscopy, it is shown that the steps up to and including formation of tetrachlorobenzoquinone are rapid (5-30/s). Before products can be released from the active site, the strongly oxidizing tetrachlorobenzoquinone abstracts an electron from a donor at the active site, possibly a cysteine residue, resulting in an off-pathway diradical state that only slowly reverts to an intermediate capable of completing the catalytic cycle | Sphingobium chlorophenolicum | 2,3,5,6-tetrachloro-1,4-benzoquinone + NADP+ + chloride + H2O | - |
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Synonyms | Comment | Organism |
---|---|---|
PCP hydroxylase | - |
Sphingobium chlorophenolicum |
pentachlorophenol hydroxylase | - |
Sphingobium chlorophenolicum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
Pentachlorophenol | pH 7.0, 22°C | Sphingobium chlorophenolicum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | flavoprotein | Sphingobium chlorophenolicum |
General Information | Comment | Organism |
---|---|---|
metabolism | degradation of pentachlorophenol | Sphingobium chlorophenolicum |