Application | Comment | Organism |
---|---|---|
degradation | pentachlorophenol is a chloroaromatic pesticide used to protect lumber, and an environmental pollutant, Sphingobium chlorophenolicum is a microorganism that can degrade the agent to 3-oxoadipate using 5 catalytic enzymes, pentachlorophenol 4-monooxygenase catalyzes the first and rate-limiting step | Sphingobium chlorophenolicum |
Cloned (Comment) | Organism |
---|---|
Escherichia coli M15 overexpressing recombinant hexahistidine-tagged pentachlorophenol 4-monooxygenase | Sphingobium chlorophenolicum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
pentachlorophenol + 2 NADPH + H+ + O2 | Sphingobium chlorophenolicum | - |
tetrachlorohydroquinone + 2 NADP+ + chloride + H2O | product is not tetrachlorobenzoquinone as previously thought, ethyl acetate and glutathione can shift the redox-equilibrium in favor of tetrachlorobenzoquinone, room temperature (23-25°C), 50 mM phosphate buffer, pH 7.0, while with NADPH tetrachlorobenzoquinone is reduced to tetrachlorohydroquinone (with 0.5-1 mol NADPH/mol tetrachlorobenzoquinone 50-65% are reduced, with 2.5 mol or more almost complete reduction occurs), deionized distilled water, pH 6.8 (due to interference of phosphate or Tris-HCl buffer with the detection of tetrachlorohydroquinone) | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sphingobium chlorophenolicum | P42535 | ATCC 39723 | - |
Purification (Comment) | Organism |
---|---|
frozen pellet of cell culture is suspended in lysis buffer (50 mM Tris-HCl, pH 7.7, 025 mM phenylmethanesulfonyl fluoride, 1 microM pepstatin A, lysozyme), cells disrupted by sonication, centrifuged, supernatant purified by affinity chromatography, mixed with Ni-NTA agarose medium, washed, packed into a column, eluted, active fractions combined and stored in buffer (20 mM phosphate buffer, pH 7.0, 0.25 mM phenylmethanesulfonyl fluoride, 5% glycerol) | Sphingobium chlorophenolicum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pentachlorophenol + 2 NADPH + H+ + O2 | - |
Sphingobium chlorophenolicum | tetrachlorohydroquinone + 2 NADP+ + chloride + H2O | product is not tetrachlorobenzoquinone as previously thought, ethyl acetate and glutathione can shift the redox-equilibrium in favor of tetrachlorobenzoquinone, room temperature (23-25°C), 50 mM phosphate buffer, pH 7.0, while with NADPH tetrachlorobenzoquinone is reduced to tetrachlorohydroquinone (with 0.5-1 mol NADPH/mol tetrachlorobenzoquinone 50-65% are reduced, with 2.5 mol or more almost complete reduction occurs), deionized distilled water, pH 6.8 (due to interference of phosphate or Tris-HCl buffer with the detection of tetrachlorohydroquinone) | ? |
Synonyms | Comment | Organism |
---|---|---|
PcpB | - |
Sphingobium chlorophenolicum |
pentachlorophenol 4-monooxygenase | - |
Sphingobium chlorophenolicum |
General Information | Comment | Organism |
---|---|---|
metabolism | hydroxylation of pentachlorophenol in the pentachlorophenol biodegradation pathway | Sphingobium chlorophenolicum |