Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) | Sphingobium chlorophenolicum |
Protein Variants | Comment | Organism |
---|---|---|
F223A | site-directed mutagenesis, increased activity compared to the wild-type enzyme | Sphingobium chlorophenolicum |
F225A | site-directed mutagenesis, increased activity compared to the wild-type enzyme | Sphingobium chlorophenolicum |
F85A | site-directed mutagenesis, 60% decreased activity compared to the wild-type enzyme | Sphingobium chlorophenolicum |
F87A | site-directed mutagenesis, mutant enzyme is nearly not soluble in contrast to the wild-type, highly reduced activity compared to the wild-type enzyme | Sphingobium chlorophenolicum |
R235E | site-directed mutagenesis, reduced activity compared to the wild-type enzyme | Sphingobium chlorophenolicum |
R235K | site-directed mutagenesis, increased activity compared to the wild-type enzyme | Sphingobium chlorophenolicum |
R235K | site-directed mutagenesis, mutant enzyme is nearly not soluble in contrast to the wild-type, reduced activity compared to the wild-type enzyme | Sphingobium chlorophenolicum |
Y216A | site-directed mutagenesis, highly reduced activity compared to the wild-type enzyme | Sphingobium chlorophenolicum |
Y216F | site-directed mutagenesis, 60% decreased activity compared to the wild-type enzyme | Sphingobium chlorophenolicum |
Y397A | site-directed mutagenesis, highly reduced activity compared to the wild-type enzyme | Sphingobium chlorophenolicum |
Y397F | site-directed mutagenesis, 60% increased activity compared to the wild-type enzyme | Sphingobium chlorophenolicum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Sphingobium chlorophenolicum | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
x * 60000, SDS-PAGE | Sphingobium chlorophenolicum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
pentachlorophenol + NADPH + H+ + O2 | Sphingobium chlorophenolicum | pentachlorophenol degradation | tetrachlorohydroquinone + NADP+ + chloride + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sphingobium chlorophenolicum | - |
strain ATCC 39723 | - |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant enzymes from Escherichia coli strain Bl21(DE3) | Sphingobium chlorophenolicum |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
pentachlorophenol + NADPH + H+ + O2 = 2,3,5,6-tetrachloro-1,4-benzoquinone + NADP+ + chloride + H2O | benzene rings of Phe85, Phe87, Tyr397, as well as Tyr216 and the positive charge of Arg235 are important for activity and stabilization of the active site, reaction mechanism | Sphingobium chlorophenolicum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pentachlorophenol + NADPH + H+ + O2 | - |
Sphingobium chlorophenolicum | tetrachlorohydroquinone + NADP+ + chloride + H2O | - |
? | |
pentachlorophenol + NADPH + H+ + O2 | pentachlorophenol degradation | Sphingobium chlorophenolicum | tetrachlorohydroquinone + NADP+ + chloride + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 60000, SDS-PAGE | Sphingobium chlorophenolicum |
More | homology modeling and model building of three-dimensional structure | Sphingobium chlorophenolicum |
Synonyms | Comment | Organism |
---|---|---|
PcpB | - |
Sphingobium chlorophenolicum |
pentachlorophenol 4-monooxygenase | - |
Sphingobium chlorophenolicum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Sphingobium chlorophenolicum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Sphingobium chlorophenolicum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Sphingobium chlorophenolicum | |
NADPH | - |
Sphingobium chlorophenolicum |