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Literature summary for 1.14.13.39 extracted from

  • Chen, Y.; Jiang, B.; Zhuang, Y.; Peng, H.; Chen, W.
    Differential effects of heat shock protein 90 and serine 1179 phosphorylation on endothelial nitric oxide synthase activity and on its cofactors (2017), PLoS ONE, 12, e0179978 .
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
heat shock protein 90 heat shock protein 90 enhances the affinity of wild-type enzyme to NADPH, L-arginine, and calmodulin but not to Ca2+ and BH4 Bos taurus

Cloned(Commentary)

Cloned (Comment) Organism
overexpression of wild-type endothelial enzyme and mutant endothelial enzyme S1179D in Escherichia coli Bos taurus

Protein Variants

Protein Variants Comment Organism
S1179D mutation augments enzyme activity and increases its sensitivity to heat shock protein 90 (Hsp90). Mutation of Ser1179 to aspartic acid plays a dominant role in changing the sensitivity of the enzyme to NADPH. Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 L-arginine + 3 NADPH + 3 H+ + 4 O2 Bos taurus endothelial nitric oxide synthase (eNOS) is responsible for maintaining systemic blood pressure, vascular remodeling and angiogenesis 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O
-
?

Organism

Organism UniProt Comment Textmining
Bos taurus P29473
-
-

Source Tissue

Source Tissue Comment Organism Textmining
aortic endothelial cell
-
Bos taurus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 L-arginine + 3 NADPH + 3 H+ + 4 O2
-
Bos taurus 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O
-
?
2 L-arginine + 3 NADPH + 3 H+ + 4 O2 endothelial nitric oxide synthase (eNOS) is responsible for maintaining systemic blood pressure, vascular remodeling and angiogenesis Bos taurus 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O
-
?

Synonyms

Synonyms Comment Organism
endothelial nitric oxide synthase
-
Bos taurus
eNOS
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Bos taurus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
assay at Bos taurus

General Information

General Information Comment Organism
physiological function endothelial nitric oxide synthase (eNOS) is responsible for maintaining systemic blood pressure, vascular remodeling and angiogenesis Bos taurus