Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli strain BL21(DE3) | Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics of heme-NO complex formation in the stopped-flow reaction | Bacillus subtilis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | a heme enzyme | Bacillus subtilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 L-arginine + 3 NADPH + 3 H+ + 4 O2 | Bacillus subtilis | - |
2 citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Bacillus subtilis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
enzyme activities with different flavodoxins, overview | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 L-arginine + 2 NADPH + 2 H+ + 2 O2 | first half reaction via intermediate Nomega-hydroxy-L-arginine | Bacillus subtilis | 2 Nomega-hydroxy-L-arginine + 2 NADP+ + 2 H2O | - |
? | |
2 L-arginine + 3 NADPH + 3 H+ + 4 O2 | - |
Bacillus subtilis | 2 citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O | - |
? | |
2 L-arginine + 3 NADPH + 3 H+ + 4 O2 | overall reaction | Bacillus subtilis | 2 citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O | - |
? | |
additional information | the bacterial NOS enzymes have no attached flavoprotein domain to reduce their heme and so must rely on unknown bacterial proteins for electrons | Bacillus subtilis | ? | - |
? | |
Nomega-hydroxy-L-arginine + NADPH + H+ + O2 | second half reaction via intermediate Nomega-hydroxy-L-arginine | Bacillus subtilis | citrulline + nitric oxide + NADP+ + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
bacterial nitric-oxide synthase | - |
Bacillus subtilis |
bNOS | - |
Bacillus subtilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
assay at | Bacillus subtilis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
flavodoxin | reduced YkuN and YkuP containing FMN, YkuN is more efficient in supporting bsNOS catalysis, Km for YkuN is 0.0016 mM, for YkuP 0.022 mM, overview | Bacillus subtilis | |
heme | - |
Bacillus subtilis | |
NADPH | - |
Bacillus subtilis |