Cloned (Comment) | Organism |
---|---|
expression of GST-tagged splicing variants alpha and beta in HEK 293 cells | Rattus norvegicus |
expression of His-tagged and GST-tagged truncated nNOS, comprising residues 1-198, in Escherichia coli | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | knock out mice deficient for the splice-isoform alpha of neuronal nitric oxide synthase display residual nitric oxide synthase activity and immunosignal | Mus musculus |
additional information | knock out mice deficient for the splice-isoform alpha of neuronal nitric oxide synthase display residual nitric oxide synthase activity and immunosignal, phenotype, overview | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | inhibition of PSD-95/nNOS interaction by the nNOSalpha beta-finger antibody | Mus musculus | |
additional information | inhibition of PSD-95/nNOS interaction by the nNOSalpha beta-finger antibody | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
x * 35000, recombinant GST-tagged truncated enzyme, SDS-PAGE | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 L-arginine + 3 NADPH + 3 H+ + 4 O2 | Mus musculus | - |
2 citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O | - |
? | |
2 L-arginine + 3 NADPH + 3 H+ + 4 O2 | Rattus norvegicus | - |
2 citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
different splice isoforms of nNOS, e.g. splicing variant nNOSalpha | - |
Rattus norvegicus | - |
different splice isoforms of nNOS, e.g. splicing variant nNOSalpha | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged or GST-tagged truncated nNOS, comprising residues 1-198, from Escherichia coli by nickel affinity or glutathione affinity chromatography, respectively | Mus musculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Mus musculus | - |
brain | - |
Rattus norvegicus | - |
cerebellum | - |
Mus musculus | - |
cerebellum | - |
Rattus norvegicus | - |
cerebral cortex | - |
Mus musculus | - |
cerebral cortex | - |
Rattus norvegicus | - |
corpus striatum | - |
Mus musculus | - |
corpus striatum | - |
Rattus norvegicus | - |
hippocampus | - |
Mus musculus | - |
hippocampus | - |
Rattus norvegicus | - |
laterodorsal tegmental nucleus | - |
Mus musculus | - |
laterodorsal tegmental nucleus | - |
Rattus norvegicus | - |
additional information | nNOS splicing variants tissue distribution, immunohistochemic analysis, overview | Mus musculus | - |
additional information | nNOS splicing variants tissue distribution, immunohistochemic analysis, overview | Rattus norvegicus | - |
neuron | neuronal NOS, nNOS | Mus musculus | - |
neuron | neuronal NOS, nNOS | Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 L-arginine + 3 NADPH + 3 H+ + 4 O2 | - |
Mus musculus | 2 citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O | - |
? | |
2 L-arginine + 3 NADPH + 3 H+ + 4 O2 | - |
Rattus norvegicus | 2 citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 35000, recombinant GST-tagged truncated enzyme, SDS-PAGE | Mus musculus |
More | the splicing variant nNOSaalpha contains a specific beta-finger motif involved in PDZ domain scaffolding | Rattus norvegicus |
Synonyms | Comment | Organism |
---|---|---|
neuronal nitric oxide synthase | - |
Mus musculus |
neuronal nitric oxide synthase | - |
Rattus norvegicus |
nNOSalpha | - |
Mus musculus |
nNOSalpha | - |
Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Mus musculus | |
NADPH | - |
Rattus norvegicus |