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Literature summary for 1.14.13.39 extracted from

  • Langnaese, K.; Richter, K.; Smalla, K.H.; Krauss, M.; Thomas, U.; Wolf, G.; Laube, G.
    Splice-isoform specific immunolocalization of neuronal nitric oxide synthase in mouse and rat brain reveals that the PDZ-complex-building nNOSalpha beta-finger is largely exposed to antibodies (2007), Dev. Neurobiol., 67, 422-437.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of GST-tagged splicing variants alpha and beta in HEK 293 cells Rattus norvegicus
expression of His-tagged and GST-tagged truncated nNOS, comprising residues 1-198, in Escherichia coli Mus musculus

Protein Variants

Protein Variants Comment Organism
additional information knock out mice deficient for the splice-isoform alpha of neuronal nitric oxide synthase display residual nitric oxide synthase activity and immunosignal Mus musculus
additional information knock out mice deficient for the splice-isoform alpha of neuronal nitric oxide synthase display residual nitric oxide synthase activity and immunosignal, phenotype, overview Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
additional information inhibition of PSD-95/nNOS interaction by the nNOSalpha beta-finger antibody Mus musculus
additional information inhibition of PSD-95/nNOS interaction by the nNOSalpha beta-finger antibody Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
x * 35000, recombinant GST-tagged truncated enzyme, SDS-PAGE Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 L-arginine + 3 NADPH + 3 H+ + 4 O2 Mus musculus
-
2 citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O
-
?
2 L-arginine + 3 NADPH + 3 H+ + 4 O2 Rattus norvegicus
-
2 citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O
-
?

Organism

Organism UniProt Comment Textmining
Mus musculus
-
different splice isoforms of nNOS, e.g. splicing variant nNOSalpha
-
Rattus norvegicus
-
different splice isoforms of nNOS, e.g. splicing variant nNOSalpha
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged or GST-tagged truncated nNOS, comprising residues 1-198, from Escherichia coli by nickel affinity or glutathione affinity chromatography, respectively Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
Mus musculus
-
brain
-
Rattus norvegicus
-
cerebellum
-
Mus musculus
-
cerebellum
-
Rattus norvegicus
-
cerebral cortex
-
Mus musculus
-
cerebral cortex
-
Rattus norvegicus
-
corpus striatum
-
Mus musculus
-
corpus striatum
-
Rattus norvegicus
-
hippocampus
-
Mus musculus
-
hippocampus
-
Rattus norvegicus
-
laterodorsal tegmental nucleus
-
Mus musculus
-
laterodorsal tegmental nucleus
-
Rattus norvegicus
-
additional information nNOS splicing variants tissue distribution, immunohistochemic analysis, overview Mus musculus
-
additional information nNOS splicing variants tissue distribution, immunohistochemic analysis, overview Rattus norvegicus
-
neuron neuronal NOS, nNOS Mus musculus
-
neuron neuronal NOS, nNOS Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 L-arginine + 3 NADPH + 3 H+ + 4 O2
-
Mus musculus 2 citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O
-
?
2 L-arginine + 3 NADPH + 3 H+ + 4 O2
-
Rattus norvegicus 2 citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O
-
?

Subunits

Subunits Comment Organism
? x * 35000, recombinant GST-tagged truncated enzyme, SDS-PAGE Mus musculus
More the splicing variant nNOSaalpha contains a specific beta-finger motif involved in PDZ domain scaffolding Rattus norvegicus

Synonyms

Synonyms Comment Organism
neuronal nitric oxide synthase
-
Mus musculus
neuronal nitric oxide synthase
-
Rattus norvegicus
nNOSalpha
-
Mus musculus
nNOSalpha
-
Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Mus musculus
NADPH
-
Rattus norvegicus