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Literature summary for 1.14.13.39 extracted from
- The role of nitric oxide in aspirin induced thrombolysis in vitro and the purification of aspirin activated nitric oxide synthase from human blood platelets (2007), Am. J. Hematol., 82, 986-995.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Acetylsalicylic acid | maximal activation at 0.004 mM | Homo sapiens |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | activates | Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 19000 | - |
gel filtration | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 L-arginine + 3 NADPH + 3 H+ + 4 O2 | Homo sapiens | NO from acetylsalicylic acid-activated enzyme is involved in thrombolysis, overview | 2 citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme from platelets activated by acetylsalicylic acid, by solubilization with Triton X-100, anion exchange chromatography, and gel filtration, to homogeneity | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| blood platelet | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 L-arginine + 3 NADPH + 3 H+ + 4 O2 | - |
Homo sapiens | 2 citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O | - |
? | |
| 2 L-arginine + 3 NADPH + 3 H+ + 4 O2 | NO from acetylsalicylic acid-activated enzyme is involved in thrombolysis, overview | Homo sapiens | 2 citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 19000, SDS-PAGE | Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Homo sapiens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | - |
Homo sapiens | |
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Release 2023.1 (January 2023)
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