Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Ca2+/calmodulin | calmodulin activates electron transfer from NADPH through three reductase domains to the oxygenase domain, controls constitutive isoforms through regulation of electron transfer between NADPH and heme | Rattus norvegicus | |
Ca2+/calmodulin | calmodulin activates electron transfer from NADPH through three reductase domains to the oxygenase domain, controls constitutive isoforms through regulation of electrontransfer between NADPH and heme | Mus musculus | |
Ca2+/calmodulin | calmodulin activates electron transfer from NADPH through three reductase domains to the oxygenase domain, controls constitutive isoforms through regulation of electrontransfer between NADPH and heme | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+/calmodulin | calmodulin activates electron transfer from NADPH through three reductase domains to the oxygenase domain, controls constitutive isoforms through regulation of electron transfer between NADPH and heme | Rattus norvegicus | |
Ca2+/calmodulin | calmodulin activates electron transfer from NADPH through three reductase domains to the oxygenase domain, controls constitutive isoforms through regulation of electrontransfer between NADPH and heme | Mus musculus | |
Ca2+/calmodulin | calmodulin activates electron transfer from NADPH through three reductase domains to the oxygenase domain, controls constitutive isoforms through regulation of electrontransfer between NADPH and heme | Homo sapiens | |
Iron | heme iron, FMN/heme electron transfer | Mus musculus | |
Iron | heme iron, FMN/heme electron transfer | Rattus norvegicus | |
Iron | heme iron, FMN/heme electron transfer | Homo sapiens | |
Zn2+ | bound by Cys104, Cys109, and Cys194 of isozyme iNOS oxygenase domain | Mus musculus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
69000 | - |
2 * 69000, iNOS, SDS-PAGE | Mus musculus |
75000 | - |
2 * 75000, nNOS, SDS-PAGE | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 L-arginine + 3 NADPH + 4 O2 + 3 H+ | Mus musculus | - |
2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O | - |
? | |
2 L-arginine + 3 NADPH + 4 O2 + 3 H+ | Rattus norvegicus | - |
2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O | - |
? | |
2 L-arginine + 3 NADPH + 4 O2 + 3 H+ | Homo sapiens | - |
2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O | - |
? | |
additional information | Mus musculus | calmodulin-controlled isoforms are signal generators, overview | ? | - |
? | |
additional information | Rattus norvegicus | calmodulin-controlled isoforms are signal generators, overview | ? | - |
? | |
additional information | Homo sapiens | calmodulin-controlled isoforms are signal generators, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P29474 | isozyme eNOS | - |
Mus musculus | - |
isozyme iNOS | - |
Rattus norvegicus | P29476 | isozyme nNOS | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
2 L-arginine + 3 NADPH + 3 H+ + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O | interaction and electrontransfer between enzyme domains and bound cofactors calmodulin, FMN, FAD, tetrahydrobiopterin, heme, and NADPH, overview | Mus musculus | |
2 L-arginine + 3 NADPH + 3 H+ + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O | interaction and electrontransfer between enzyme domains and bound cofactors calmodulin, FMN, FAD, tetrahydrobiopterin, heme, and NADPH, overview | Rattus norvegicus | |
2 L-arginine + 3 NADPH + 3 H+ + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O | interaction and electrontransfer between enzyme domains and bound cofactors calmodulin, FMN, FAD, tetrahydrobiopterin, heme, and NADPH, overview | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Rattus norvegicus | - |
endothelium | - |
Homo sapiens | - |
macrophage | - |
Mus musculus | - |
neuron | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 L-arginine + 3 NADPH + 4 O2 + 3 H+ | - |
Mus musculus | 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O | - |
? | |
2 L-arginine + 3 NADPH + 4 O2 + 3 H+ | - |
Rattus norvegicus | 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O | - |
? | |
2 L-arginine + 3 NADPH + 4 O2 + 3 H+ | - |
Homo sapiens | 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O | - |
? | |
additional information | calmodulin-controlled isoforms are signal generators, overview | Mus musculus | ? | - |
? | |
additional information | calmodulin-controlled isoforms are signal generators, overview | Rattus norvegicus | ? | - |
? | |
additional information | calmodulin-controlled isoforms are signal generators, overview | Homo sapiens | ? | - |
? | |
additional information | both oxyFMN and oxygenase domain activity are measured by following H2O2-supported oxidation of Nomega-hydroxy-L-Arg, L-NOHA, overview | Mus musculus | ? | - |
? | |
additional information | both oxyFMN and oxygenase domain activity are measured by following H2O2-supported oxidation of Nomega-hydroxy-L-Arg, L-NOHA, overview | Rattus norvegicus | ? | - |
? | |
additional information | both oxyFMN and oxygenase domain activity are measured by following H2O2-supported oxidation of Nomega-hydroxy-L-Arg, L-NOHA, overview | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Homo sapiens |
dimer | 2 * 69000, iNOS, SDS-PAGE | Mus musculus |
dimer | 2 * 75000, nNOS, SDS-PAGE | Rattus norvegicus |
Synonyms | Comment | Organism |
---|---|---|
endothelial nitric oxide synthase | - |
Homo sapiens |
eNOS | - |
Homo sapiens |
inducible nitric oxide synthase | - |
Mus musculus |
iNOS | - |
Mus musculus |
neuronal nitric oxide synthase | - |
Rattus norvegicus |
nNOS | - |
Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Mus musculus |
37 | - |
assay at | Rattus norvegicus |
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
assay at | Mus musculus |
7.6 | - |
assay at | Rattus norvegicus |
7.6 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Mus musculus | |
FAD | - |
Rattus norvegicus | |
FAD | - |
Homo sapiens | |
FMN | FMN/heme electron transfer, FMN is capable of serving as a one electron heme reductant | Mus musculus | |
FMN | FMN/heme electron transfer, FMN is capable of serving as a one electron heme reductant | Rattus norvegicus | |
FMN | FMN/heme electron transfer, FMN is capable of serving as a one electron heme reductant | Homo sapiens | |
NADPH | - |
Mus musculus | |
NADPH | - |
Rattus norvegicus | |
NADPH | - |
Homo sapiens |