Cloned (Comment) | Organism |
---|---|
inducible liver isoform is expressed in Escherichia coli with and without His-tag, requires coexpression of calmodulin | Homo sapiens |
General Stability | Organism |
---|---|
inducible isoform is unstable during purification in absence of L-arginine and tetrahydrobiopterin toward loss of the heme group and formation of low-spin species | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.022 | - |
L-arginine | in presence of tetrahydrobiopterin and L-arginine | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | no stimulation with exogenous Ca2+, inducible isoform from liver | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
inducible | - |
Purification (Comment) | Organism |
---|---|
recombinant wild-type of inducible liver isoform from Escherichia coli with and without His-tag, requires inclusion of tetrahydrobiopterin in purification buffer | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | inducible isoform | Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
38 | - |
purified enzyme, inducible isoform, cytochrome c reductase activity | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 L-arginine + 3 NADPH + 4 O2 + 3 H+ | - |
Homo sapiens | 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
Calmodulin | no stimulation with exogenous calmodulin, inducible isoform from liver | Homo sapiens | |
FAD | no flavin | Homo sapiens | |
FMN | no flavin | Homo sapiens |