Activating Compound | Comment | Organism | Structure |
---|---|---|---|
interferon gamma | activates | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | no inhibition | Mus musculus | |
ethylene glycol bis(beta-amino-ethylether)-N,N,N',N'-tetraacetic acid | i.e. EGTA, complete inhibition of cytosolic enzyme, partial inhibition of particulate enzyme | Mus musculus | |
additional information | the macrophage enzyme is not inhibited by calmodulin antagonists (N-4-aminobutyl-), (N-6-aminohexyl)-5-chloro-2-naphthalene sulfonamide | Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0003 | - |
NADPH | - |
Mus musculus | |
0.002 | 0.0028 | L-arginine | - |
Mus musculus | |
0.0028 | - |
L-arginine | - |
Mus musculus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | slight activation | Mus musculus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
dissociates irreversibly into subunits in the absence of L-arginine, FAD and tetrahydrobiopterin | Mus musculus |
130000 | - |
2 * 130000, SDS-PAGE | Mus musculus |
250000 | - |
gel filtration | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 L-arginine + 3 NADPH + 4 O2 + 3 H+ | Mus musculus | enzyme of mammalian immune, cardiovascular and neural systems, synthesizing the free radical nitric oxide or a NO-releasing product | 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
gel filtration chromatography | Mus musculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
macrophage | Ca2+-independent form | Mus musculus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.06 | - |
purified enzyme | Mus musculus |
Storage Stability | Organism |
---|---|
-80°C, diluted preparation stable overnight | Mus musculus |
4°C, 6 h stable | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 L-arginine + 3 NADPH + 4 O2 + 3 H+ | - |
Mus musculus | 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O | - |
? | |
2 L-arginine + 3 NADPH + 4 O2 + 3 H+ | enzyme of mammalian immune, cardiovascular and neural systems, synthesizing the free radical nitric oxide or a NO-releasing product | Mus musculus | 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 130000, SDS-PAGE | Mus musculus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Mus musculus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
t1/2: about 6 h | Mus musculus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
5,6,7,8-tetrahydro-L-biopterin | - |
Mus musculus | |
Calmodulin | murine macrophage enzyme is Ca2+/calmodulin independent | Mus musculus | |
FAD | 2.2 mol FAD per mol of enzyme dimer | Mus musculus | |
FAD | non-covalently bound FAD | Mus musculus | |
FMN | 1.1 mol FMN per mol enzyme dimer | Mus musculus | |
NADPH | - |
Mus musculus |